Hit or miss in phylogeographic analyses: the case of the cryptic NUMTs

Authors

  • CORALIE BERTHEAU,

    1. Institute of Forest Entomology, Forest Pathology and Forest Protection, Boku, University of Natural Resources and Life Sciences, Hasenauerstrasse 38, Vienna, Austria
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  • HANNES SCHULER,

    1. Institute of Forest Entomology, Forest Pathology and Forest Protection, Boku, University of Natural Resources and Life Sciences, Hasenauerstrasse 38, Vienna, Austria
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  • SUSANNE KRUMBÖCK,

    1. Institute of Forest Entomology, Forest Pathology and Forest Protection, Boku, University of Natural Resources and Life Sciences, Hasenauerstrasse 38, Vienna, Austria
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  • WOLFGANG ARTHOFER,

    1. Molecular Ecology Group, University of Innsbruck, Technikerstrasse 25, 6020 Innsbruck, Austria
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    • Equally contributing last authors.

  • CHRISTIAN STAUFFER

    1. Institute of Forest Entomology, Forest Pathology and Forest Protection, Boku, University of Natural Resources and Life Sciences, Hasenauerstrasse 38, Vienna, Austria
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Coralie Bertheau, Fax: +43 1 368635297; E-mail: coralie.bertheau@boku.ac.at

Abstract

Phylogeographic studies call for attention as nuclear copies of mitochondrial DNA (NUMT) may generate erroneous results. Here, we report the presence of NUMTs differing only by 1–3 bp from authentic mitochondrial haplotypes, consequently named cryptic NUMTs. In contrast to traditional NUMTs, for which reliable tools for detection are established, cryptic NUMTs question the validity of phylogeographic analyses based solely on mitochondrial DNA, like the one presented here on the European bark beetle Ips typographus. Caution is called as cryptic NUMTs might be responsible for haplotype richness found in several species, and the necessity of refined methods for NUMT detection is highlighted.

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