Present address: Museum of Archaeology, University of Stavanger, NO-4036 Stavanger, Norway.
Use of allele-specific sequencing primers is an efficient alternative to PCR subcloning of low-copy nuclear genes
Article first published online: 21 SEP 2011
© 2011 Blackwell Publishing Ltd
Molecular Ecology Resources
Volume 12, Issue 1, pages 128–135, January 2012
How to Cite
SCHEEN, A.-C., PFEIL, B. E., PETRI, A., HEIDARI, N., NYLINDER, S. and OXELMAN, B. (2012), Use of allele-specific sequencing primers is an efficient alternative to PCR subcloning of low-copy nuclear genes. Molecular Ecology Resources, 12: 128–135. doi: 10.1111/j.1755-0998.2011.03070.x
- Issue published online: 16 DEC 2011
- Article first published online: 21 SEP 2011
- Received 28 March 2011; revision received 29 July 2011; accepted 15 August 2011
Table S1 List of homoeologs recovered using the allele-specific primers of this study including information regarding specimen vouchers, which homoeologs were recovered using which of the allele-specific primers and corresponding Genbank accession numbers.
Fig. S1 A comparison of chromatograms resulting from direct sequencing using the original PCR primer and the allele-specific sequencing primer of decreasing length. The specificity of the primer is increased as the primer length is reduced, and the sequencing results converge on the targeted allele.
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