Microsatellite resources for Passeridae species: a predicted microsatellite map of the house sparrow Passer domesticus

Authors

  • DEBORAH A. DAWSON,

    1. Department of Animal and Plant Sciences, University of Sheffield, Sheffield S10 2TN, UK
    2. Department of Biology, University of Leicester, Leicester LE1 7RH, UK
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  • GAVIN J. HORSBURGH,

    1. Department of Animal and Plant Sciences, University of Sheffield, Sheffield S10 2TN, UK
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  • ANDREW P. KRUPA,

    1. Department of Animal and Plant Sciences, University of Sheffield, Sheffield S10 2TN, UK
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  • IAN R. K. STEWART,

    1. Department of Animal and Plant Sciences, University of Sheffield, Sheffield S10 2TN, UK
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    • Present addresses: Department of Biology, University of Delaware, Newark, DE 19716, USA.

  • SIGRUN SKJELSETH,

    1. Department of Animal and Plant Sciences, University of Sheffield, Sheffield S10 2TN, UK
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    • Department of Biology, Centre for Conservation Biology, Norwegian University of Science and Technology, NO-7491 Trondheim, Norway.

  • HENRIK JENSEN,

    1. Department of Animal and Plant Sciences, University of Sheffield, Sheffield S10 2TN, UK
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    • Department of Biology, Centre for Conservation Biology, Norwegian University of Science and Technology, NO-7491 Trondheim, Norway.

  • ALEXANDER D. BALL,

    1. Department of Animal and Plant Sciences, University of Sheffield, Sheffield S10 2TN, UK
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    • Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK.

  • LEWIS G. SPURGIN,

    1. Department of Animal and Plant Sciences, University of Sheffield, Sheffield S10 2TN, UK
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    • School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK.

  • MARIA-ELENA MANNARELLI,

    1. Department of Animal and Plant Sciences, University of Sheffield, Sheffield S10 2TN, UK
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  • SHINICHI NAKAGAWA,

    1. Department of Animal and Plant Sciences, University of Sheffield, Sheffield S10 2TN, UK
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    • Department of Zoology, University of Otago, PO Box 56, 9054 Dunedin, New Zealand.

  • JULIA SCHROEDER,

    1. Department of Animal and Plant Sciences, University of Sheffield, Sheffield S10 2TN, UK
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  • CARL VANGESTEL,

    1. Department of Animal and Plant Sciences, University of Sheffield, Sheffield S10 2TN, UK
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    • Terrestrial Ecology Unit, Ghent University, K.L. Ledeganckstraat 35, 9000 Ghent, Belgium.

  • GAVIN N. HINTEN,

    1. Department of Animal and Plant Sciences, University of Sheffield, Sheffield S10 2TN, UK
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    • Department of Sustainability, Environment, Water, Population and Communities, GPO Box 787, Canberra, ACT, Australia.

  • TERRY BURKE

    1. Department of Animal and Plant Sciences, University of Sheffield, Sheffield S10 2TN, UK
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Deborah A. Dawson, Fax: +44 (0)114 222 0002; E-mail: d.a.dawson@sheffield.ac.uk

Abstract

We identified microsatellite sequences of potential utility in the house sparrow (Passer domesticus) and assigned their predicted genome locations. These sequences included newly isolated house sparrow loci, which we fully characterized. Many of the newly isolated loci were polymorphic in two other species of Passeridae: Berthelot’s pipit Anthus berthelotii and zebra finch Taeniopygia guttata. In total, we identified 179 microsatellite markers that were either isolated directly from, or are of known utility in, the house sparrow. Sixty-seven of these markers were designed from unique sequences that we isolated from a house sparrow genomic library. These new markers were combined with 36 house sparrow markers isolated by other studies and 76 markers isolated from other passerine species but known to be polymorphic in the house sparrow. We utilized sequence homology to assign chromosomal locations for these loci in the assembled zebra finch genome. One hundred and thirty-four loci were assigned to 25 different autosomes and eight loci to the Z chromosome. Examination of the genotypes of known-sex house sparrows for 37 of the new loci revealed a W-linked locus and an additional Z-linked locus. Locus Pdoμ2, previously reported as autosomal, was found to be Z-linked. These loci enable the creation of powerful and cost-effective house sparrow multiplex primer sets for population and parentage studies. They can be used to create a house sparrow linkage map and will aid the identification of quantitative trait loci in passerine species.

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