Present addresses: Department of Biology, University of Delaware, Newark, DE 19716, USA.
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Microsatellite resources for Passeridae species: a predicted microsatellite map of the house sparrow Passer domesticus
Article first published online: 9 FEB 2012
DOI: 10.1111/j.1755-0998.2012.03115.x
© 2012 Blackwell Publishing Ltd
Additional Information
How to Cite
DAWSON, D. A., HORSBURGH, G. J., KRUPA, A. P., STEWART, I. R. K., SKJELSETH, S., JENSEN, H., BALL, A. D., SPURGIN, L. G., MANNARELLI, M.-E., NAKAGAWA, S., SCHROEDER, J., VANGESTEL, C., HINTEN, G. N. and BURKE, T. (2012), Microsatellite resources for Passeridae species: a predicted microsatellite map of the house sparrow Passer domesticus. Molecular Ecology Resources, 12: 501–523. doi: 10.1111/j.1755-0998.2012.03115.x
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Present addresses: Department of Biology, University of Delaware, Newark, DE 19716, USA.
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Department of Biology, Centre for Conservation Biology, Norwegian University of Science and Technology, NO-7491 Trondheim, Norway.
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Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK.
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School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK.
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Department of Zoology, University of Otago, PO Box 56, 9054 Dunedin, New Zealand.
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Terrestrial Ecology Unit, Ghent University, K.L. Ledeganckstraat 35, 9000 Ghent, Belgium.
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Department of Sustainability, Environment, Water, Population and Communities, GPO Box 787, Canberra, ACT, Australia.
Publication History
- Issue published online: 9 APR 2012
- Article first published online: 9 FEB 2012
- Received 8 June 2010; revision received 9 December 2011; accepted 14 December 2011
Keywords:
- Aves;
- genome map;
- microsatellite;
- Passeridae;
- passerine;
- sex-linked loci
Abstract
We identified microsatellite sequences of potential utility in the house sparrow (Passer domesticus) and assigned their predicted genome locations. These sequences included newly isolated house sparrow loci, which we fully characterized. Many of the newly isolated loci were polymorphic in two other species of Passeridae: Berthelot’s pipit Anthus berthelotii and zebra finch Taeniopygia guttata. In total, we identified 179 microsatellite markers that were either isolated directly from, or are of known utility in, the house sparrow. Sixty-seven of these markers were designed from unique sequences that we isolated from a house sparrow genomic library. These new markers were combined with 36 house sparrow markers isolated by other studies and 76 markers isolated from other passerine species but known to be polymorphic in the house sparrow. We utilized sequence homology to assign chromosomal locations for these loci in the assembled zebra finch genome. One hundred and thirty-four loci were assigned to 25 different autosomes and eight loci to the Z chromosome. Examination of the genotypes of known-sex house sparrows for 37 of the new loci revealed a W-linked locus and an additional Z-linked locus. Locus Pdoμ2, previously reported as autosomal, was found to be Z-linked. These loci enable the creation of powerful and cost-effective house sparrow multiplex primer sets for population and parentage studies. They can be used to create a house sparrow linkage map and will aid the identification of quantitative trait loci in passerine species.