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Table S1 Details of 144 GoldenGate SNP assays developed from the Antarctic fur seal transcriptome assembly (see Materials and methods for details). ‘Chromosome in the dog’ refers to the genomic location inferred by mapping each isotig to the dog (Canis familaris) genome. Basic Local Alignment Search Tool (BLAST) results indicate the top match of each isotig to the non-redundant (nr) database. Gene Ontology (GO) codes are given only for isotigs that recovered functional annotations relating to growth or immunity. In silico Minor Allele Frequency (MAF) and depth of coverage refer to the exact site of each SNP and are given only for reads accepted as unambiguous by the program SWAP454 (Brockman et al. 2008). Assay Design Tool (ADT) scores vary between 0 and 1, with values of 0.6 or above indicating a high probability of conversion into a successful genotyping assay.

Table S2 Polymorphism characteristics of 102 polymorphic SNP assays in 440 Antarctic fur seals individuals (see Materials and methods for details). The GenTrain score takes into account the quality, shape and degree of separation of the genotype clusters, with higher values indicating improved clustering (Fan et al. 2003).

FilenameFormatSizeDescription
MEN_3158_sm_TableS1.xlsx42KSupporting info item
MEN_3158_sm_TableS2.xlsx20KSupporting info item

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