Figure S1. Maximum likelihood phylogenetic tree generated from recorded rps16 haplotype sequences and sister-group taxa using MEGA 5.0 (Tamura et  al., 2011)*1.

Figure S2. Correlation between HRM Tm calculation in-vitro and in-silico.

Figure S3. In-silico simulation of SNP mutation sensitivity for HRM analysis using Arenaria ciliata rps16I haplotype I01 as a wild-type template (352 bp length).

Table S1. Location and size of sample populations of Arenaria ciliata (Ac) and A.  norvegica (An) used in High Resolution Melt Analysis.

Table S2. Results of in silico RFLP analysis carried out using NEBcutter (Vincze et  al. 2003).

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