Rapid identification of chloroplast haplotypes using High Resolution Melting analysis
Version of Record online: 11 JUL 2012
© 2012 Blackwell Publishing Ltd
Molecular Ecology Resources
Volume 12, Issue 5, pages 894–908, September 2012
How to Cite
DANG, X.-D., KELLEHER, C. T., HOWARD-WILLIAMS, E. and MEADE, C. V. (2012), Rapid identification of chloroplast haplotypes using High Resolution Melting analysis. Molecular Ecology Resources, 12: 894–908. doi: 10.1111/j.1755-0998.2012.03164.x
- Issue online: 16 AUG 2012
- Version of Record online: 11 JUL 2012
- Received 16 February 2012; revision received 27 April 2012; accepted 4 May 2012
Figure S1. Maximum likelihood phylogenetic tree generated from recorded rps16 haplotype sequences and sister-group taxa using MEGA 5.0 (Tamura et al., 2011)*1.
Figure S2. Correlation between HRM Tm calculation in-vitro and in-silico.
Figure S3. In-silico simulation of SNP mutation sensitivity for HRM analysis using Arenaria ciliata rps16I haplotype I01 as a wild-type template (352 bp length).
Table S1. Location and size of sample populations of Arenaria ciliata (Ac) and A. norvegica (An) used in High Resolution Melt Analysis.
Table S2. Results of in silico RFLP analysis carried out using NEBcutter (Vincze et al. 2003).
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