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Fig. S1 Regulation of BIM expression though mRNA abundance in human melanoma cells. Total RNA was extracted from the WM35 (RGP), WM793 (VGP) and WM9 (Met) melanoma cell lines following treatment with 2 μM CI-1040 (in DMSO) for 24 hours. mRNA expression was assessed by real-time PCR as described in Materials and Methods. Expression data are shown for the genes BIM and MCL-1. Graphs indicate the abundance of mRNA levels relative to those of cells treated with DMSO alone (a value arbitrarily set to 1) for duplicate samples expressed as mean ± SEM.

Fig. S2 Regulation of BCL-2 family protein expression by trophic factor deprivation or MEK inhibition in WM793 cells. Asynchronously growing WM793 cells were treated with either 2 μM CI-1040 alone, subjected to TFD, or both 2 μM CI-1040 and TFD combined for 24 hours or 48 hours as indicated. Cells were lysed and lysates analysed by immunoblotting with antibodies which recognize BIM, MCL-1, BCL-2 and ERK1/ERK2 total proteins or phosphorylated ERK1/ERK2.

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