These authors contributed equally to this work.
Oncogenic BRAFV600E inhibits BIM expression to promote melanoma cell survival
Article first published online: 28 JUL 2008
© 2008 The Authors, Journal Compilation © 2008 Blackwell Munksgaard
Pigment Cell & Melanoma Research
Volume 21, Issue 5, pages 534–544, October 2008
How to Cite
Cartlidge, R. A., Thomas, G. R., Cagnol, S., Jong, K. A., Molton, S. A., Finch, A. J. and McMahon, M. (2008), Oncogenic BRAFV600E inhibits BIM expression to promote melanoma cell survival. Pigment Cell & Melanoma Research, 21: 534–544. doi: 10.1111/j.1755-148X.2008.00491.x
- Issue published online: 18 SEP 2008
- Article first published online: 28 JUL 2008
- PUBLICATION DATA Received 10 March 2008, revised and accepted for publication 8 July 2008
Fig. S1 Regulation of BIM expression though mRNA abundance in human melanoma cells. Total RNA was extracted from the WM35 (RGP), WM793 (VGP) and WM9 (Met) melanoma cell lines following treatment with 2 μM CI-1040 (in DMSO) for 24 hours. mRNA expression was assessed by real-time PCR as described in Materials and Methods. Expression data are shown for the genes BIM and MCL-1. Graphs indicate the abundance of mRNA levels relative to those of cells treated with DMSO alone (a value arbitrarily set to 1) for duplicate samples expressed as mean ± SEM.
Fig. S2 Regulation of BCL-2 family protein expression by trophic factor deprivation or MEK inhibition in WM793 cells. Asynchronously growing WM793 cells were treated with either 2 μM CI-1040 alone, subjected to TFD, or both 2 μM CI-1040 and TFD combined for 24 hours or 48 hours as indicated. Cells were lysed and lysates analysed by immunoblotting with antibodies which recognize BIM, MCL-1, BCL-2 and ERK1/ERK2 total proteins or phosphorylated ERK1/ERK2.
Please note: Wiley-Blackwell are not responsible for the content or functionality of any supporting materials supplied by the authors. Any queries (other than missing material) should be directed to the corresponding author for the article.
|PCMR_491_sm_SupplementalFigures.ppt||455K||Supporting info item|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.