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The Wellcome Trust Functional Genomics Cell Bank: holdings

Article first published online: 20 OCT 2009

DOI: 10.1111/j.1755-148X.2009.00641.x

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Pigment Cell & Melanoma Research

Pigment Cell & Melanoma Research

Volume 23, Issue 1, pages 147–150, February 2010

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(2010), The Wellcome Trust Functional Genomics Cell Bank: holdings. Pigment Cell & Melanoma Research, 23: 147–150. doi: 10.1111/j.1755-148X.2009.00641.x

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  1. Issue published online: 12 JAN 2010
  2. Article first published online: 20 OCT 2009

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This cell bank specializes in mouse and human melanocyte, melanoblast and melanoma lines, though a variety of other cell types are held. There are mutant mouse melanocytes of around 40 genotypes. Cells are freely supplied to researchers, subject to an MTA in some cases, or permission from the source if elsewhere. There is a small fee; researchers with very limited finances can enquire about a waiver of this.

More information at the above web site, which also gives the culture medium recommended for each cell line, melanocyte culture methods, and sources of materials.

Mouse melanocytes, progenitors and melanomas

Donor mouse genotypeCell lineStrain backgroundReference
Mouse melanocyte lines: black
Nonagouti (a/a), otherwise wild-type melan-aC57BL/6JBennett et al., 1987
melan-a2C57BL/6JSviderskaya et al., 1995
melan-a3, a4C57BL/6JSviderskaya et al., 2002
DMEL-21, DMEL-31C57BL/6JPrince et al., 2001
Ink4a-Arf (Cdkn2a) null2, a/amelan-Ink4a-Arf1 to 4C57BL/6JSviderskaya et al., 2002
Ink4a-Arf +/−, a/amelan-Ink4a-Arf+/−C57BL/6JSviderskaya et al., 2002
Ink4a-Arf +/−, a/amelan-a5 to a7Mixed strain a/aHida et al., 2009
Ink4a (p16) null, a/amelan-Ink4a-1 to 4C57BL/6JHa et al., 2007
Arf null, a/amelan-Arf-1, -Arf-2C57BL/6JHa et al., 2007
p53 (Tp53) null, a/amelan-p53-1 to -3C57BL/6JHa et al., 2007
Mouse melanocyte lines: other pigmentary genotypes. Alphabetical by locus/mutation name
Agouti (A/A)melan-AC3HPalmisano et al., 2008
Mel-181, Mel-291C3HDe Sepulveda et al., 1994
Dominant yellow (Ay/a)melan-Ay1 to -Ay33C57BL/6JHida et al., 2009
Ashen (Rab27ash/Rab27ash)melan-ash1 to -ash33C57BL/6JAli et al., 2004
Brown (Tyrp1b/Tyrp1b)melan-bQ strainBennett et al., 1989
melan-b-HmRQ strainWakeling et al., 1992
melan-Ink4a-Arf-b1 to -b33C57BL/6JSviderskaya et al., 2002
Belted (Adamts20bt/Adamts20bt)melan-bt1 to bt31,3C57BL/6JUnpublished
Albino (Tyrc/Tyrc)melan-cMF1Bennett et al., 1989
melan-c2 to -c41,3C57BL/6JPlowright, 2007
Himalayan (Tyrc-h/Tyrc-h)melan-c-h1 to -c-h31,3C57BL/6JPlowright, 2007
Chocolate (Rab38cht/Rab38cht)melan-cht1 to cht73C57BL/6JWasmeier et al., 2006
Cocoa (Hps3coa/Hps3coa)melan-coaC57BL/10JSuzuki et al., 2001
ep, Pale ear (Hps1ep/Hps1ep)melan-ep1, -ep23C57BL/6JSuzuki et al., 2002
Grey-lethal (Ostm1gl/Ostm1gl)melan-gl11, -gl21B6/129SvUnpublished
Gunmetal (Rabggtagm/Rabggtagm)melan-gm11C57BL/6JUnpublished
Light ear (Hps4le/Hps4le)melan-le1, -le23C57BL/6JSuzuki et al., 2002
Leaden (Myo5aln/Myo5aln)melan-ln1 to -ln43C57BL/6JHume et al., 2006
Mahoganoid null (Mgrn1md-nc/ Mgrn1md-nc)melan-md1, -md24Mixed strain a/aHida et al., 2009
Mahogany null (Atrnmg−3J/ Atrnmg−3J) melan-mg1, -mg24Mixed strain a/aHida et al., 2009
Melanocortin 1 receptor (Mc1re/Mc1re) melan-e1, to -e33C57BL/6JHida et al., 2009
Misty (Dock7m/Dock7m)melan-m1, to -m3C57BL/6JSviderskaya et al., 1998b
Mocha (Ap3dmh/Ap3dmh)melan-mh1, -mh21C57BL/6JUnpublished
Muted (Mumu/Mumu)melan-mu1 to -mu33B6/CHMU/LeSetty et al., 2007
Ocular albinism 1 KO (Oa1−/−, Oa1+/−)melan-Oa1, melan-Oa1+/−C3HPalmisano et al., 2008
Pallid (Pldnpa/Pldnpa)melan-pa1, -pa23C57BL/6JSetty et al., 2007
Pearl (Ap3b1pe/Ap3b1pe)melan-pe1C57BL/6JTheos et al., 2005
Pinkeyed dilution null (Oca2p-cp/Oca2p−25H)melan-p1, -p2C3HSviderskaya et al., 1997
Pinkeyed dilution null (Oca2p-J/Oca2p-J)melan-p5, -p71, 3C57BL/6JPlowright, 2007
Reduced pigmentation (Bloc1s3rp/ Bloc1s3rp)melan-rp3 to -rp53C57BL/6JGwynn et al., 2004
Recessive spotting (rs/rs)melan-rsC57BL/6JSpanakis et al., 1992
Piebald spotting (Ednrbs/Ednrbs)melan-s1C3HSviderskaya et al., 1998a
Silver (Sisi/Sisi)melan-si3 to -si53C57BL/6JTheos et al., 2006
Silver, heterozygous brown (Sisi/Sisi, Tyrp1+/Tyrp1b)melan-si1, -si2C57BL/6JSpanakis et al., 1992
Subtle grey (Slc7a11sut/Slc7a11sut)melan-sut1 to -sut33B6/C3H/HeSnJChintala et al., 2005
Upstream factor KO (Usf-/-)melan-Usf1MixedCorre et al., 2004
Mouse melanoblast lines
Nonagouti (a/a) blackmelb-aC57BL/6JSviderskaya et al., 1995
Piebald spotting (Ednrbs/Edrbs)melb-s1 to -s3C3HSviderskaya et al., 1998a
Pinkeyed dilution null (pcp/p25H)melb-p3, -p4C3HSviderskaya et al., 1997
Misty (Dock7m/Dock7m)melb-m5C57BL/6JSviderskaya et al., 1998b
Neural crest-like cell lines
Misty (Dock7m/Dock7m)NC-m4, NC-m6C57BL/6JSviderskaya et al., 2009
Mouse melanoma cell lines
Nonagouti (a/a), blackB16-C3C57BL/6JKreider et al., 1975
B16-C3.3, B16 C3.6C57BL/6JBennett, 1983
B16-F11, B16-F101C57BL/6JFidler, 1975
A/AK1735-m21C3H/HeNFidler et al., 1981

Other cell lines

Cell lineNotesReference
Human immortal melanocytes
Note: Grown under low-level biohazard containment conditions as they contain disabled amphotropic viruses used in the immortalization procedure.
Hermes 11, Hermes 21From p16 null patients. Animal experiments with Hermes 2 not permitted. Immortalized with hTERT (neo vector) only. Hermes 1 is immortalized from primary strain Hmel-p16-1, and Hermes 2 from Hmel-p16-2. Sublines: Hermes 2a (slightly aneuploid), Hermes 2b and 2c (both diploid).Sviderskaya et al., 2003
Hermes 31Immortalized lines from Nohm-1 normal diploid human melanocyte strain. Sublines: Hermes 3a has hTERT (puro) and CDK4 (neo) expression vectors. Hermes 3b has hTERT (puro) and antisense p16 (neo) vectors. Hermes 3c has hTERT (puro) and HPV16-E7 (neo) vectors.Gray-Schopfer et al., 2006
Hermes 41Immortalized lines from 830c normal diploid melanocytes. 830c melanocytes carry two variant MC1R alleles: R160W/D294H. Sublines: Hermes 4a has hTERT (puro) and CDK4 (neo) expression vectors. Hermes 4b has hTERT (puro) and antisense p16 (neo) vectors. Hermes 4c has hTERT (puro) and HPV16-E7 (neo) vectors.Gray-Schopfer et al., 2006;  Scott et al., 2002
Human melanoma cell lines (metastatic unless stated)
A375PParental, metastaticKozlowski et al., 1984
A375MSubline of A375P, highly metastatic in miceKozlowski et al., 1984
COLO 6971Easty et al., 1995a
COLO 7921Easty et al., 1995a
COLO 7931No publication known
COLO 8001Easty et al., 1995a
COLO 8181Easty et al., 1995a
COLO 8321$$ = lines from different secondary sites from the same patient.Maung et al., 1999
COLO 8391$COLO 839: In-transit line, grown from peripheral bloodMaung et al., 1999
COLO 8451$Morse and Moore, 1993
COLO 8571$Maung et al., 1999
COLO 8581$Maung et al., 1999
DX31Parental, metastaticDracopoli et al., 1987
DX3-LT5.11Subline, highly metastatic in miceOrmerod et al., 1986
LT5.1 HmR1Hygromycin-resistant version of DX3-LT5.1Unpublished
ME 14021Primary, VGPLarizza et al., 1989
ME 105381Primary, VGPLarizza et al., 1989
MM961TgRThioguanine-resistant subline of MM96Pope et al., 1979 (MM96); Unpublished subline
MM2001Primary, ?VGPPope et al., 1979
MM4851McEwan et al., 1989
RPMI 79321Quinn et al., 1977
SK-MEL-231Houghton et al., 1987
SK-MEL-23-neoR1G418-resistantUnpublished subline
WM-91Balaban et al., 1984
WM-9-HmR1Hygromycin-resistantUnpublished subline
WM-351Primary, RGPHerlyn, 1990
WM-98-11Primary, VGPHerlyn et al., 1985b
WM-1641Herlyn D. et al., 1990
WM-239A1Herlyn et al., 1985a
WM-239A1HmRHygromycin-resistantUnpublished subline
WM-8521Herlyn M. et al., 1990
WM-11581Easty et al., 1995b
WM-1341B1Primary, VGPHerlyn, 1990
WM-16501Primary, RGPEasty et al., 1995b
451LU1More-metastatic subline of WM-164Herlyn D. et al., 1990
Other mouse skin cells
XB2 (subline)Immortal keratinocytes, derived from a teratocarcinoma. Our subline selected to grow without fibroblast feeder cellsBennett et al., 1989; Rheinwald and Green, 1975
SC1Dermal fibroblast-like line with growth-stimulatory properties for melanoblastsSviderskaya et al., 1995
Fibro-Ink4a-ArfDermal fibroblasts from Ink4a-Arf null miceSviderskaya et al., 2002
Fibro-AyDermal fibroblasts from Ay/A mice, expected to secrete ASPHida et al., 2009
Other holdings
In brief, the bank also holds other differentiating cell types: mouse embryonal carcinoma, rat pluripotent neuroblastoma cells, mouse myoblast lines, and rat mammary stem cells. It also has rat mammary epithelial, myoepithelial and fibroblastic lines, other mouse fibroblast lines, and various retroviral producer lines. See web site for details.

References

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    Direct Link:
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  • Corre, S., Primot, A., Sviderskaya, E., Bennett, D.C., Vaulont, S., Goding, C.R., and Galibert, M.D. (2004). UV-induced expression of key component of the tanning process, the POMC and MC1R genes, is dependent on the p-38-activated upstream stimulating factor-1 (USF-1). J. Biol. Chem. 279, 51226–51233.
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  • Easty, D.J., Guthrie, B.A., Maung, K., Farr, C.J., Lindberg, R.A., Toso, R.J., Herlyn, M., and Bennett, D.C. (1995a). Protein B61 as a new growth factor: expression of B61 and up-regulation of its receptor epithelial cell kinase during melanoma progression. Cancer Res. 55, 2528–2532.
  • Easty, D.J., Herlyn, M., and Bennett, D.C. (1995b). Abnormal protein tyrosine kinase gene expression during melanoma progression and metastasis. Int. J. Cancer 60, 129–136.
    Direct Link:
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  • Gray-Schopfer, V.C., Cheong, S.C., Chow, J., Moss, A., Abdel-Malek, Z.A., Marais, R., Wynford-Thomas, D., and Bennett, D.C. (2006). Cellular senescence in naevi and immortalisation in melanoma: a role for p16? Br. J. Cancer 95, 496–505.
  • Gwynn, B., Martina, J.A., Bonifacino, J.S. et al. (2004). Reduced pigmentation (rp), a mouse model of Hermansky-Pudlak syndrome, encodes a novel component of the BLOC-1 complex. Blood 104, 3181–3189.
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  • Suzuki, T., Li, W., Zhang, Q., Novak, E.K., Sviderskaya, E.V., Wilson, A., Bennett, D.C., Roe, B.A., Swank, R.T., and Spritz, R.A. (2001). The gene mutated in cocoa mice, carrying a defect of organelle biogenesis, is a homologue of the human Hermansky-Pudlak Syndrome-3 gene. Genomics 78, 30–37.
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  • Sviderskaya, E.V., Hill, S.P., Evans-Whipp, T.J., Chin, L., Orlow, S.J., Easty, D.J., Cheong, S.C., Beach, D., DePinho, R.A., and Bennett, D.C. (2002). p16Ink4a in melanocyte senescence and differentiation. J. Natl Cancer Inst. 94, 446–454.
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