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Figure S1. (A) Lysates from melanocytes (MC) cultured from black (n = 3) and white (n = 6) foreskins were blotted for FP receptor. FP receptor expression was higher in white, compared with black, melanocytes. (B) Quantitative real time PCR show that white melanocytes expressed 1.9 × 10−11 ng/ml (SD ± × 10−12) compared with black melanocytes, which expressed 2.2 × 10−10 ng/ml (SD ± 1.4 × 10−10). (C) The human melanoma cell line WM115, and human melanocytes, were plated in basal media in the top well of a 96 well migration chamber (QCM Chemotaxis Cell Migration kit). PGF2&agr; was placed in the bottom well and twenty-four hours later, cells that had migrated were quantitated as per manufacturer's instructions. PGF2&agr; had no effect on melanoma or melanocyte migration, even at high doses.

Table S1. Lysates from MC cultured from black (pooled cultures from three individual foreskins) and white (pooled cultures from six individual foreskins) foreskins were resolved on 10% SDS-PAGE and blotted for FP receptor.

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FilenameFormatSizeDescription
PCMR_661_sm_figS1AB.tif1273KSupporting info item
PCMR_661_sm_figS1C.tif2059KSupporting info item
PCMR_661_sm_tableS1.tif1561KSupporting info item

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.