MC1R stimulation by α-MSH induces catalase and promotes its re-distribution to the cell periphery and dendrites

Authors

  • Vittoria Maresca,

    1. Laboratorio di Fisiopatologia Cutanea e Biologia Molecolare-Centro di Metabolomica, San Gallicano Dermatologic Institute IRCCS, Rome, Italy
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  • Enrica Flori,

    1. Laboratorio di Fisiopatologia Cutanea e Biologia Molecolare-Centro di Metabolomica, San Gallicano Dermatologic Institute IRCCS, Rome, Italy
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  • Barbara Bellei,

    1. Laboratorio di Fisiopatologia Cutanea e Biologia Molecolare-Centro di Metabolomica, San Gallicano Dermatologic Institute IRCCS, Rome, Italy
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  • Nicaela Aspite,

    1. Laboratorio di Fisiopatologia Cutanea e Biologia Molecolare-Centro di Metabolomica, San Gallicano Dermatologic Institute IRCCS, Rome, Italy
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  • Daniela Kovacs,

    1. Laboratorio di Fisiopatologia Cutanea e Biologia Molecolare-Centro di Metabolomica, San Gallicano Dermatologic Institute IRCCS, Rome, Italy
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  • Mauro Picardo

    1. Laboratorio di Fisiopatologia Cutanea e Biologia Molecolare-Centro di Metabolomica, San Gallicano Dermatologic Institute IRCCS, Rome, Italy
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Mauro Picardo, e-mail: picardo@ifo.itVittoria Maresca and Enrica Flori contributed equally to this work.

Summary

We demonstrated a direct correlation between melanogenic and catalase activities on in vitro and ex vivo models. Here, we investigated whether the stimulation of Melanocortin-1 Receptor (MC1R) could influence catalase expression, activity and cellular localization. For this purpose, we treated B16-F0 melanoma cells with α-Melanocyte Stimulating Hormone (α-MSH) and we showed a rapid induction of catalase through a cAMP/PKA-dependent, microphthalmia-associated transcription factor (MITF) independent mechanism, acting at post-transcriptional level. Moreover, α-MSH promoted a partial re-distribution of catalase to the cell periphery and dendrites. This work strengthens the correlation between melanogenesis and anti-oxidants, demonstrating the induction of catalase in response to a melanogenic stimulation, such as α-MSH-dependent MC1R activation. Moreover, this study highlights catalase regulatory mechanisms poorly known, and attributes to α-MSH a protective role in defending melanocytes, and possibly keratinocytes, not only on the basis of its pigmentary action, but also for its capacity to stimulate a quick anti-oxidant defence.

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