SEARCH

SEARCH BY CITATION

Figure S1. A. Characterisation of POU3F3 binding sites at the Mitf-m locus. A. DNaseI footprinting. The protected regions FP1-FP2 are indicated. B. Sequence of the Mitf-m promoter showing the boxed TATA element, the CRE in green, the GATA element in blue and the two POU3F2 binding sites in red. The alignment of the FP1 and FP2 regions with the NORE and 5′-CATNNNTAAT-3′ motifs is also shown.

Figure S2. POU3F2 occupancy of sites in the Mitf-j and Mitf-b promoters. Graphic representation of the HA ChIP-chip results on 501Mel cells expressing 3HA-POU3F2 in the UCSC web browser at the indicated loci. Binding peaks are shown by arrows.

Figure S3. Characterisation of POU3F3 binding sites at the Rarb, Fgf7 and Bmi loci. The upper part of each panel shows the graphic representation of the ChIP-chip results in the UCSC browser. Below is the DNaseI 3HA-POU3F2 Mitf-m locus. A. DNaseI footprinting of the amplified region under the peaks where the protected regions are indicated. The sequences of the protected regions along with their alignment with the known POU3F2 binding motifs are shown below the footprinting panel. The relative affinity of each sequenced based on the efficiency of protection in the footprinting experiments is indicated from low + to high +++.

Figure S4. Characterisation of POU3F3 binding sites at the Kcnj2, and Lrrn3 loci. The layout of the figure is as in Supplemental Figure 3.

Table S1. POU3F2 bound genes in 501Mel cells. Excel table of annotated loci bound by POU3F2. Loci bound by POU3F2 with gene symbol, RefSeq transcript, normalised ratio, peak score, chromosome location and Agilent identifier of the peak oligonucleotide and location of binding sites with respect to TSS.

Table S2. Page 1 shows the ontology analysis of POU3F2-occupied genes performed by David (http://david.abcc.ncifcrf.gov/). In addition to the list of genes in each identified functional category, are shown the number of genes (gene count) the % of the total number of genes, and pvalue. Page 2 shows the KEGG pathway analysis of the POU3F2-occupied genes.

Table S3. Affymetrix transcriptome data. Page 1 of the Excel file shows the total data set from the POU3F2 overexpression and negative control. The signal averages from the cells transfected with CMV-POU3F2 and CMV control are indicated along with the fold and log2 change values and the gene and transcript identifiers. Page 2 shows the same data for the upregulated genes.

Please note: Wiley-Blackwell are not responsible for the content or functionality of any supporting materials supplied by the authors. Any queries (other than missing material) should be directed to the corresponding author for the article.

FilenameFormatSizeDescription
PCMR_697_sm_apps1.doc34KSupporting info item
PCMR_697_sm_fs1.jpg497KSupporting info item
PCMR_697_sm_fs2.jpg475KSupporting info item
PCMR_697_sm_fs3.jpg749KSupporting info item
PCMR_697_sm_fs4.jpg772KSupporting info item
PCMR_697_sm_tabs1.xls405KSupporting info item
PCMR_697_sm_tabs2.xls51KSupporting info item
PCMR_697_sm_tabs3.xls8407KSupporting info item

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.