Allele-specific genetic interactions between Mitf and Kit affect melanocyte development
Version of Record online: 29 MAR 2010
© 2010 John Wiley & Sons A/S
Pigment Cell & Melanoma Research
Volume 23, Issue 3, pages 441–447, June 2010
How to Cite
Wen, B., Chen, Y., Li, H., Wang, J., Shen, J., Ma, A., Qu, J., Bismuth, K., Debbache, J., Arnheiter, H. and Hou, L. (2010), Allele-specific genetic interactions between Mitf and Kit affect melanocyte development. Pigment Cell & Melanoma Research, 23: 441–447. doi: 10.1111/j.1755-148X.2010.00699.x
- Issue online: 12 MAY 2010
- Version of Record online: 29 MAR 2010
- PUBLICATION DATA Received 17 July 2009, revised and accepted for publication 20 March 2010, published online 29 March 2010
Figure S1. A. The Mitfmi-bws/Kit interaction is Kit-specific as no interaction is seen between Mitfmi-bws and Ednrbtm1Myks, an Ednrb null allele. B. No interaction between Kittm1Alf and MitfS73A. Note white feet characteristic of the Kittm1Alf/+ genotype but no extensive spotting, unlike what is seen in Mitfmi-bws/+; Kittm1Alf/+ mice.
Figure S2. RT-PCR using RNA from black dorsal skin of C57BL/6 wild type (wt), Mitfmi-bws/mi-bws (mi-bws/mi-bws), and Kittm1Alf/+ mice. Primers mapped to Mitf or Usf1 (upstream stimulatory factor-1) exons as indicated. Note that wt contains more M-Mitf exon 2B+ compared to M-Mitf exon 2B- cDNA while the M-Mitf exon 2B+/2B- cDNA ratio is reversed in mi-bws/mi-bws (primers 1M-3). The total levels of M-Mitf cDNA is, however, similar for wt and mi-bws/mi-bws skin and only lower for Kittm1Alf/+ skin for which, because of the lower levels, it becomes difficult to assess the relative 2B+/2B- ratio. Total Mitf cDNA levels (measured using primers 6B-8, amplifying Mitf from melanocytes and the remainder of skin tissue), are only slightly lower in mi-bws/mi-bws or Kittm1Alf/+ skin compared to wt. Note similar levels of control Usf1 cDNA for all samples.
Figure S3. Real time RT-PCR for the tissue samples and genotypes indicated. Separate quantitation was performed for exon 2B+ and exon 2B- cDNA, using primers giving equal length products as described in Bharti et al., in press. A. Wild type, and heterozygous and homozygous single-gene mutant genotypes. B. Wild type and Mitfmi-vga9/S73A compound mutants either lacking or carrying Kittm1Alf.
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