Downregulation of SIK2 expression promotes the melanogenic program in mice
Article first published online: 24 SEP 2010
© 2010 John Wiley & Sons A/S
Pigment Cell & Melanoma Research
Volume 23, Issue 6, pages 809–819, December 2010
How to Cite
Horike, N., Kumagai, A., Shimono, Y., Onishi, T., Itoh, Y., Sasaki, T., Kitagawa, K., Hatano, O., Takagi, H., Susumu, T., Teraoka, H., Kusano, K.-i., Nagaoka, Y., Kawahara, H. and Takemori, H. (2010), Downregulation of SIK2 expression promotes the melanogenic program in mice. Pigment Cell & Melanoma Research, 23: 809–819. doi: 10.1111/j.1755-148X.2010.00760.x
- Issue published online: 13 OCT 2010
- Article first published online: 24 SEP 2010
- Accepted manuscript online: 31 AUG 2010 09:32AM EST
- PUBLICATION DATA Received 12 July 2010, revised and accepted for publication 27 August 2010, published online 31 August 2010
Figure S1. (A) Examples of mice with different hair color and their respective genotypes. (B) Examples of matings and their offspring. As Ay/Ay mice were lethal, the mating on the right produces offspring with the same genotype as their parents.
Figure S2. (A) Strategies to produce SIK2-knockout mice. (B) Southern blot analysis of mouse DNA with different SIK2 genotypes. (C) PCR analysis of mouse DNA. (D) Confirmation of the loss of SIK2 protein in SIK2-knockout mice. As SIK2 is abundantly expressed in white adipose tissue (WAT) or brown adipose tissue (BAT), we isolated these tissues and performed immunoprecipitation followed by Western blot analyses.
Figure S3. Strategy to introduce the SIK2-/- genotype into Ay/a mice. Representative F2 mice (4-weeks-old) are shown (left).
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