p53 prevents progression of nevi to melanoma predominantly through cell cycle regulation
Version of Record online: 13 OCT 2010
© 2010 John Wiley & Sons A/S
Pigment Cell & Melanoma Research
Volume 23, Issue 6, pages 781–794, December 2010
How to Cite
Terzian, T., Torchia, E. C., Dai, D., Robinson, S. E., Murao, K., Stiegmann, R. A., Gonzalez, V., Boyle, G. M., Powell, M. B., Pollock, P. M., Lozano, G., Robinson, W. A., Roop, D. R. and Box, N. F. (2010), p53 prevents progression of nevi to melanoma predominantly through cell cycle regulation. Pigment Cell & Melanoma Research, 23: 781–794. doi: 10.1111/j.1755-148X.2010.00773.x
- Issue online: 13 OCT 2010
- Version of Record online: 13 OCT 2010
- Accepted manuscript online: 16 SEP 2010 11:37PM EST
- PUBLICATION DATA Received 12 May 2010, revised and accepted for publication 13 September 2010, published online 16 September 2010
Figure S1. p53 and Ki67 immunofluorescent staining of nevi and melanomas. (A) p53 immunostaining (green) in TP-ras0/+ and TP-ras0/+: Mdm4+/− mice. S100 (red) detects melanocytes. Brightfield panels shows the pigmentation status of the corresponding stained section. (B) Ki67 staining (green) in nevus versus melanoma, with S100 (red) used as the melanocyte marker.
Figure S2. Hematoxylin and eosin (HE) staining after melanin bleaching of nevi and melanoma. Melanoma (right panel) is characterized by presence of atypical epitheliod melanocytes that have cytologic atypia manifesting as pleomorphism (different sized cells). Abnormal chromatin (AC) patterns are present including dense staining of nuclear patches, as well as thickened nuclear membranes (NM) and large abnormally shaped eosinophilic nucleoli (N), and spontaneous apoptotic cells (Ap).
Figure S3. Relative expression levels measured by qPCR of few genes found up or down-regulated by microarray analysis. CC, Cell cycle regulator; ECM, extra-cellular matrix.
Table S1. Characterization TPRAS0/+ and TPRAS0/+: Mdm4+/− mice and tumors.
Table S2. Mutations identified in used melanoma cell lines.
Table S3. Up and down regulated genes after 18 h of Nutlin 3 treatment.
Table S4. Up and down regulated genes after 72 h of Nutlin 3 treatment.
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