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Figure S1. p53 and Ki67 immunofluorescent staining of nevi and melanomas. (A) p53 immunostaining (green) in TP-ras0/+ and TP-ras0/+: Mdm4+/− mice. S100 (red) detects melanocytes. Brightfield panels shows the pigmentation status of the corresponding stained section. (B) Ki67 staining (green) in nevus versus melanoma, with S100 (red) used as the melanocyte marker.

Figure S2. Hematoxylin and eosin (HE) staining after melanin bleaching of nevi and melanoma. Melanoma (right panel) is characterized by presence of atypical epitheliod melanocytes that have cytologic atypia manifesting as pleomorphism (different sized cells). Abnormal chromatin (AC) patterns are present including dense staining of nuclear patches, as well as thickened nuclear membranes (NM) and large abnormally shaped eosinophilic nucleoli (N), and spontaneous apoptotic cells (Ap).

Figure S3. Relative expression levels measured by qPCR of few genes found up or down-regulated by microarray analysis. CC, Cell cycle regulator; ECM, extra-cellular matrix.

Table S1. Characterization TPRAS0/+ and TPRAS0/+: Mdm4+/− mice and tumors.

Table S2. Mutations identified in used melanoma cell lines.

Table S3. Up and down regulated genes after 18 h of Nutlin 3 treatment.

Table S4. Up and down regulated genes after 72 h of Nutlin 3 treatment.

FilenameFormatSizeDescription
PCMR_773_sm_figS1.pdf30394KSupporting info item
PCMR_773_sm_fS2.eps11925KSupporting info item
PCMR_773_sm_fS3.eps768KSupporting info item
PCMR_773_sm_TableS1.doc59KSupporting info item
PCMR_773_sm_TableS2.doc39KSupporting info item
PCMR_773_sm_TableS3S4.xls774KSupporting info item

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