Figure S1. A375 cells were transfected with expression constructs encoding either GFP alone or a GFP-OTC fusion protein (A and B, respectively). Transfection efficiencies were similar judging by the number of green fluorescent cells observed (right panel). (C) MTT assay showed that GFP-OTC-transfected A375 cells were significantly less sensitive to rhArg-mediated growth inhibition after a 3-day treatment, when compared against overexpression of GFP alone or mock transfection without plasmid (n = 3, data plotted as means ± SEM).

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