These authors contributed equally.
Melanoma cell invasiveness is regulated by miR-211 suppression of the BRN2 transcription factor
Article first published online: 7 APR 2011
© 2011 John Wiley & Sons A/S
Pigment Cell & Melanoma Research
Volume 24, Issue 3, pages 525–537, June 2011
How to Cite
Boyle, G. M., Woods, S. L., Bonazzi, V. F., Stark, M. S., Hacker, E., Aoude, L. G., Dutton-Regester, K., Cook, A. L., Sturm, R. A. and Hayward, N. K. (2011), Melanoma cell invasiveness is regulated by miR-211 suppression of the BRN2 transcription factor. Pigment Cell & Melanoma Research, 24: 525–537. doi: 10.1111/j.1755-148X.2011.00849.x
- Issue published online: 16 MAY 2011
- Article first published online: 7 APR 2011
- Accepted manuscript online: 17 MAR 2011 10:06AM EST
- PUBLICATION DATA Received 29 November 2010, revised and accepted for publication 14 March 2011, published online 17 March 2011
To identify microRNAs potentially involved in melanomagenesis, we compared microRNA expression profiles between melanoma cell lines and cultured melanocytes. The most differentially expressed microRNA between the normal and tumor cell lines was miR-211. We focused on this pigment-cell-enriched miRNA as it is derived from the microphthalmia-associated transcription factor (MITF)-regulated gene, TRPM1 (melastatin). We find that miR-211 expression is greatly decreased in melanoma cells and melanoblasts compared to melanocytes. Bioinformatic analysis identified a large number of potential targets of miR-211, including POU3F2 (BRN2). Inhibition of miR-211 in normal melanocytes resulted in increased BRN2 protein, indicating that endogenous miR-211 represses BRN2 in differentiated cells. Over-expression of miR-211 in melanoma cell lines changed the invasive potential of the cells in vitro through directly targeting BRN2 translation. We propose a model for the apparent non-overlapping expression levels of BRN2 and MITF in melanoma, mediated by miR-211 expression.