These authors contributed equally.
Melanoma cell invasiveness is regulated by miR-211 suppression of the BRN2 transcription factor
Version of Record online: 7 APR 2011
© 2011 John Wiley & Sons A/S
Pigment Cell & Melanoma Research
Volume 24, Issue 3, pages 525–537, June 2011
How to Cite
Boyle, G. M., Woods, S. L., Bonazzi, V. F., Stark, M. S., Hacker, E., Aoude, L. G., Dutton-Regester, K., Cook, A. L., Sturm, R. A. and Hayward, N. K. (2011), Melanoma cell invasiveness is regulated by miR-211 suppression of the BRN2 transcription factor. Pigment Cell & Melanoma Research, 24: 525–537. doi: 10.1111/j.1755-148X.2011.00849.x
- Issue online: 16 MAY 2011
- Version of Record online: 7 APR 2011
- Accepted manuscript online: 17 MAR 2011 10:06AM EST
- PUBLICATION DATA Received 29 November 2010, revised and accepted for publication 14 March 2011, published online 17 March 2011
Figure S1. (A) Quantitative Real time-PCR validation of relative miR-211 expression levels in normal human melanocytes and melanoma cell lines used in this study. (B) Melanoblasts (MB) from two different neonate foreskins (Donors 2 & 3) were independently differentiated into melanocytes (MB:MC) as previously described (Cook et al., 2003), before determination of BRN2, MITFand TRPM1 levels.
Figure S2. Relative miR-211 expression levels following lentiviral transduction to generate polyclonal cell lines.
Figure S3. Potential miR-211 binding sites in the BRN2 3’UTR are conserved across species.
Figure S4. Mutation of Site 3 results in a fairly minimal derepression of the BRN2 3’ UTR reporter.
Figure S5. Diagram showing the human BRN2 3’UTR.
Table S1. Summary of predicted miR-211 & miR-204 targets: source of prediction, miRNA binding site information, method of validation attempted.
Table S2. Local free energy scores in BRN2 mRNA for potential miR-211 binding sites.
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