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Figure S1. (A) Quantitative Real time-PCR validation of relative miR-211 expression levels in normal human melanocytes and melanoma cell lines used in this study. (B) Melanoblasts (MB) from two different neonate foreskins (Donors 2 & 3) were independently differentiated into melanocytes (MB:MC) as previously described (Cook et al., 2003), before determination of BRN2, MITFand TRPM1 levels.

Figure S2. Relative miR-211 expression levels following lentiviral transduction to generate polyclonal cell lines.

Figure S3. Potential miR-211 binding sites in the BRN2 3’UTR are conserved across species.

Figure S4. Mutation of Site 3 results in a fairly minimal derepression of the BRN2 3’ UTR reporter.

Figure S5. Diagram showing the human BRN2 3’UTR.

Table S1. Summary of predicted miR-211 & miR-204 targets: source of prediction, miRNA binding site information, method of validation attempted.

Table S2. Local free energy scores in BRN2 mRNA for potential miR-211 binding sites.

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PCMR_849_sm_fs1.tif23643KSupporting info item
PCMR_849_sm_fs2.tif10519KSupporting info item
PCMR_849_sm_fs3.tif367KSupporting info item
PCMR_849_sm_fs4.tif13966KSupporting info item
PCMR_849_sm_fs5.tif311KSupporting info item
PCMR_849_sm_tables1.doc56KSupporting info item
PCMR_849_sm_tables2.doc29KSupporting info item

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