Junctional adhesion molecules are required for melanoma cell lines transendothelial migration in vitro

Authors

  • Stephanie Ghislin,

    1.  Team ‘Régulation des Réponses Immunitaires’, Institut Jacques Monod CNRS-Université Paris Diderot, Bâtiment Buffon, 15 rue Hélène Brion, 75205 Paris, Cedex 13, France
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  • Dorian Obino,

    1.  Team ‘Régulation des Réponses Immunitaires’, Institut Jacques Monod CNRS-Université Paris Diderot, Bâtiment Buffon, 15 rue Hélène Brion, 75205 Paris, Cedex 13, France
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  • Sandrine Middendorp,

    1.  Team ‘Régulation des Réponses Immunitaires’, Institut Jacques Monod CNRS-Université Paris Diderot, Bâtiment Buffon, 15 rue Hélène Brion, 75205 Paris, Cedex 13, France
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  • Nicole Boggetto,

    1.  Imagoseine, plate-forme de cytométrie en flux, Institut Jacques Monod CNRS-Université Paris Diderot, Paris, France
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  • Catherine Alcaide-Loridan,

    1.  Team ‘Régulation des Réponses Immunitaires’, Institut Jacques Monod CNRS-Université Paris Diderot, Bâtiment Buffon, 15 rue Hélène Brion, 75205 Paris, Cedex 13, France
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  • Frederique Deshayes

    1.  Team ‘Régulation des Réponses Immunitaires’, Institut Jacques Monod CNRS-Université Paris Diderot, Bâtiment Buffon, 15 rue Hélène Brion, 75205 Paris, Cedex 13, France
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F. Deshayes, e-mail: deshayes.frederique@ijm.univ-paris-diderot.fr

Summary

One of the main steps of metastasis is extravasation, a phenomenon well described in lymphocytes but remaining to be fully uncovered for melanoma. Junctional adhesion molecules (JAMs) control the transendothelial migration of leukocytes. To date, the role of the JAM proteins, notably JAM-A and JAM-C, has not been examined in melanoma. Here, we compared two melanoma tumor cell lines, A375 and SLM8 cells, the A375 cell line being four times more efficient than the SLM8 cells in the crossing of the endothelial monolayer. We show evidence of the differential expression of JAM-A and JAM-C in these cell lines with JAM-C mainly expressed in the A375 cell line, and JAM-A detected preferentially in the SLM8 cells. To further dissect the respective roles of these proteins, we used both siRNA and blocking antibodies to decrease JAM-A and JAM-C expression.

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