Correction added after online publication January 2012: The fifth author Samuel Gehrke was added after the original Early View publication of this paper.
Carcinogen treatment in mouse selectively expressing activated N-RasQ61K in melanocytes recapitulates metastatic cutaneous melanoma development
Article first published online: 6 JAN 2012
© 2011 John Wiley & Sons A/S
Pigment Cell & Melanoma Research
Volume 25, Issue 2, pages 275–278, March 2012
How to Cite
Contassot, E., Jankovic, D., Schuler, P., Preynat-Seauve, O., Gehrke, S., Kerl, K., Beermann, F. and French, L. E. (2012), Carcinogen treatment in mouse selectively expressing activated N-RasQ61K in melanocytes recapitulates metastatic cutaneous melanoma development. Pigment Cell & Melanoma Research, 25: 275–278. doi: 10.1111/j.1755-148X.2011.00944.x
- Issue published online: 22 FEB 2012
- Article first published online: 6 JAN 2012
- Accepted manuscript online: 29 NOV 2011 10:47AM EST
- PUBLICATION DATA Received 6 June 2011, revised and accepted for publication 28 November 2011, published online 29 November 2011
- metastatic potential
The incidence of melanoma has significantly increased, and a better understanding of its pathogenesis and development of new therapeutic strategies are urgently needed. Here, we describe a murine model of metastatic cutaneous melanoma using C57BL/6 mice expressing a mutated human N-Ras gene under the control of a tyrosinase promoter (TyrRas). These mice were topically exposed to 7,12- dimethylbenzanthracene (DMBA) for brief exposure periods. Cutaneous melanoma developed at the site of exposure on average by 19 weeks of age and in 80% of mice. Importantly, as in humans, melanoma development was associated with subsequent metastasis to tumor-draining lymph nodes. Critically, such metastatic behavior is transplantable, as intradermal inoculation of melanoma cells from TyrRas-DMBA mice into non-transgenic mice led to the growth of melanoma and, again, metastasis to skin-draining lymph nodes. This metastatic melanoma model closely mimics human pathology and should be a useful tool for studying melanoma pathogenesis and developing new therapies.