ORIGINAL ARTICLE

Proteasome inhibition and ROS generation by 4-nerolidylcatechol induces melanoma cell death

  1. Carla A. Brohem1,
  2. Renato R. Massaro1,
  3. Manoela Tiago1,
  4. Camila E. Marinho1,
  5. Miriam G. Jasiulionis2,
  6. Rebeca L. de Almeida1,
  7. Diogo P. Rivelli1,
  8. Renata C. Albuquerque1,
  9. Tiago F. de Oliveira1,
  10. Ana P. de Melo Loureiro1,
  11. Sabrina Okada1,
  12. María S. Soengas3,
  13. Silvia B. de Moraes Barros1,
  14. Silvya S. Maria-Engler1

Article first published online: 27 MAR 2012

DOI: 10.1111/j.1755-148X.2012.00992.x

Issue

Pigment Cell & Melanoma Research

Pigment Cell & Melanoma Research

Volume 25, Issue 3, pages 354–369, May 2012

Additional Information

How to Cite

Brohem, C. A., Massaro, R. R., Tiago, M., Marinho, C. E., Jasiulionis, M. G., de Almeida, R. L., Rivelli, D. P., Albuquerque, R. C., de Oliveira, T. F., de Melo Loureiro, A. P., Okada, S., Soengas, M. S., de Moraes Barros, S. B. and Maria-Engler, S. S. (2012), Proteasome inhibition and ROS generation by 4-nerolidylcatechol induces melanoma cell death. Pigment Cell & Melanoma Research, 25: 354–369. doi: 10.1111/j.1755-148X.2012.00992.x

Author Information

  1. 1

     Department of Clinical Chemistry & Toxicology, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil

  2. 2

     Pharmacology Department, Federal University of São Paulo, São Paulo, Brazil

  3. 3

     Centro Nacional de Investigaciones Oncológicas (Spanish National Cancer Research Center) Madrid, Spain

*Silvya S. Maria-Engler, e-mail: silvya@usp.br

Publication History

  1. Issue published online: 17 APR 2012
  2. Article first published online: 27 MAR 2012
  3. Accepted manuscript online: 28 FEB 2012 12:35PM EST
  4. PUBLICATION DATA Received 5 May 2011, revised and accepted for publication 17 February 2012, published online 28 February 2012

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Figure S1. Fibroblasts are resistant to 4-NC action. Human dermal fibroblasts derived from foreskin (FF282, FF284, FF287 and HNF) were treated with 4-NC (10 and 30 μM) for 24 h.

Figure S2. Cell viability measured by Trypan Blue assay.

Figure S3. Evaluation of anti and proapoptotic factors levels in melanocytes, keratinocytes and melanomas at lower concentration of 4-NC.

Figure S4. Cell viability measured by MTT assay.

Figure S5. Effects of 4-NC on the dermal equivalent contraction (normal human fibroblasts (FHN) embedded in type I collagen gel).

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PCMR_992_sm_figs3.tif7375KSupporting info item
PCMR_992_sm_figs4.tif10199KSupporting info item
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