These authors contributed equally to this work.
Lycorine hydrochloride inhibits metastatic melanoma cell-dominant vasculogenic mimicry
Article first published online: 28 AUG 2012
© 2012 John Wiley & Sons A/S
Pigment Cell & Melanoma Research
Volume 25, Issue 5, pages 630–638, September 2012
How to Cite
Liu, R., Cao, Z., Tu, J., Pan, Y., Shang, B., Zhang, G., Bao, M., Zhang, S., Yang, P. and Zhou, Q. (2012), Lycorine hydrochloride inhibits metastatic melanoma cell-dominant vasculogenic mimicry. Pigment Cell & Melanoma Research, 25: 630–638. doi: 10.1111/j.1755-148X.2012.01036.x
- Issue published online: 28 AUG 2012
- Article first published online: 28 AUG 2012
- Accepted manuscript online: 10 JUL 2012 11:14AM EST
- PUBLICATION DATA Received 13 January 2012, revised and accepted for publication 8 July 2012, published online 10 July 2012
- vasculogenic mimicry;
- lycorine hydrochloride;
Melanoma cells actively participate in tumor angiogenesis and vasculogenic mimicry. However, anti-angiogenic therapy in patients with melanoma has not shown a significant survival gain. Thus, new anti-melanoma angiogenic and vasculogenic drugs are highly desired. Using the metastatic melanoma cell line C8161 as a model, we explored melanoma vasculogenic inhibitors and found that lycorine hydrochloride (LH) effectively suppressed C8161 cell-dominant formation of capillary-like tubes in vitro and generation of tumor blood vessels in vivo with low toxicity. Mechanistic studies revealed that LH markedly hindered expression of VE-cadherin in C8161 cells, but did not affect expression of six other important angiogenic and vasculogenic genes. Luciferase assays showed that LH significantly impeded promoter activity of the VE-cadherin gene in a dose-dependent manner. Together, these data suggest that LH inhibits melanoma C8161 cell-dominant vasculogenic mimicry by reducing VE-cadherin gene expression and diminishing cell surface exposure of the protein.