The last two authors share senior authorship.
Vitreous and serum levels of vascular endothelial growth factor and platelet-derived growth factor and their correlation in patients with non-proliferative diabetic retinopathy and clinically significant macula oedema
Article first published online: 2 OCT 2009
© 2009 The Authors. Journal compilation © 2009 Acta Ophthalmol
Volume 89, Issue 3, pages 248–254, May 2011
How to Cite
Praidou, A., Papakonstantinou, E., Androudi, S., Georgiadis, N., Karakiulakis, G. and Dimitrakos, S. (2011), Vitreous and serum levels of vascular endothelial growth factor and platelet-derived growth factor and their correlation in patients with non-proliferative diabetic retinopathy and clinically significant macula oedema. Acta Ophthalmologica, 89: 248–254. doi: 10.1111/j.1755-3768.2009.01661.x
- Issue published online: 2 OCT 2009
- Article first published online: 2 OCT 2009
- Received on May 31st, 2008. Accepted on April 13th, 2009.
- clinical significant macular oedema;
- diabetic retinopathy;
- macular oedema;
- platelet-derived growth factor;
- vascular endothelial growth factor
Purpose: To investigate possible correlations between vitreous and/or serum levels of platelet-derived growth factor isoforms (PDGF-AA, -AB and -BB) with parameters associated with non-proliferative diabetic retinopathy (NPDR) and clinically significant macula oedema (CSMO); to compare the results to relevant results regarding vascular endothelial growth factor (VEGF), which is an established growth factor affecting NPDR.
Methods: Fifteen patients with NPDR, 31 patients with proliferative diabetic retinopathy (PDR) and 15 non-diabetic patients were included in the study. Vitreous and serum samples were obtained during vitrectomy. PDGF-AA, -AB and -BB, as well as VEGF, were measured by enzyme-linked immunosorbent assay (ELISA).
Results: PDGF-AA, -AB and -BB and VEGF were all expressed in the serum and vitreous of controls and patients with NPDR. The levels of PDGF-AA, PDGF-AB and VEGF in vitreous were significantly increased in the NPDR group compared to controls, while PDGF-BB levels were significantly decreased in the NPDR group compared to controls. The levels of all PDGF isoforms and VEGF in vitreous were significantly increased in the PDR group compared to the NPDR group. No such differences were evident in serum. PDGF-AA and VEGF correlated significantly to the severity of NPDR. PDGF or VEGF in vitreous of NPDR patients did not correlate with retinal photocoagulation (RP) or the serum levels of haemoglobin A1c (HbA1c). There was no correlation between the vitreous and serum levels of VEGF or PDGF in patients with PDR. Only PDGF-AB vitreous levels correlated significantly with PDGF-BB vitreous levels in the NPDR group.
Conclusion: It appears that in addition to VEGF, almost all PDGF isoforms in the vitreous are also correlated with NPDR and CSMO.