Circulating bone-marrow-derived endothelial precursor cells contribute to neovascularization in diabetic epiretinal membranes
Article first published online: 18 SEP 2009
© 2009 The Authors. Journal compilation © 2009 Acta Ophthalmol
Volume 89, Issue 3, pages 222–228, May 2011
How to Cite
Abu El-Asrar, A. M., Struyf, S., Verbeke, H., Van Damme, J. and Geboes, K. (2011), Circulating bone-marrow-derived endothelial precursor cells contribute to neovascularization in diabetic epiretinal membranes. Acta Ophthalmologica, 89: 222–228. doi: 10.1111/j.1755-3768.2009.01700.x
- Issue published online: 18 SEP 2009
- Article first published online: 18 SEP 2009
- Received on March 15th, 2009. Accepted on June 15th, 2009.
- diabetic retinopathy;
- endothelial progenitor cells;
- stromal cell-derived factor-1;
Purpose: The role of vasculogenesis, recruitment and differentiation of circulating bone-marrow-derived endothelial precursor cells into mature endothelium in proliferative diabetic retinopathy (PDR) remains undefined. We investigated the presence of bone-marrow-derived endothelial precursor cells and the expression of the chemotactic pathway SDF-1/CXCL12−CXCR4 in PDR epiretinal membranes.
Methods: Membranes from eight patients with active PDR and nine patients with inactive PDR were studied by immunohistochemistry using antibodies against CD133, vascular endothelial growth factor receptor-2 (VEGFR-2), CD14, SDF-1 and CXCR4.
Results: Blood vessels expressed CD133, VEGFR-2, CD14, SDF-1 and CXCR4 in 10, 10, 10, seven and seven out of 17 membranes, respectively. There were significant correlations between number of blood vessels expressing CD34 and number of blood vessels expressing CD133 (rs = 0.646; p = 0.005), VEGFR-2 (rs = 0.704; p = 0.002), CD14 (rs = 0.564; p = 0.018) and SDF-1 (rs = 0.577; p = 0.015). Stromal cells in close association with blood vessels expressed CD133, VEGFR-2, CD14 and CXCR4 in 10, 12, 13 and 14 membranes, respectively. The number of blood vessels expressing CD133 (p = 0.013), VEGFR-2 (p = 0.005), CD14 (p = 0.008) and SDF-1 (p = 0.005), and stromal cells expressing CD133 (p = 0.003), VEGFR-2 (p = 0.013) and CD14 (p = 0.002) was significantly higher in active membranes than in inactive membranes.
Conclusion: Bone-marrow-derived CD133+ endothelial progenitor cells and CD14+ monocytes may contribute to vasculogenesis in PDR.