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Differential effects of TGF-β and FGF-2 on in vitro proliferation and migration of primate retinal endothelial and Müller cells

  1. Phillip Romo1,2,
  2. Michele C. Madigan1,3,
  3. Jan M. Provis4,
  4. Karen M. Cullen2

Article first published online: 29 JUL 2010

DOI: 10.1111/j.1755-3768.2010.01968.x

Issue

Acta Ophthalmologica

Acta Ophthalmologica

Volume 89, Issue 3, pages e263–e268, May 2011

Additional Information

How to Cite

Romo, P., Madigan, M. C., Provis, J. M. and Cullen, K. M. (2011), Differential effects of TGF-β and FGF-2 on in vitro proliferation and migration of primate retinal endothelial and Müller cells. Acta Ophthalmologica, 89: e263–e268. doi: 10.1111/j.1755-3768.2010.01968.x

Author Information

  1. 1

    Save Sight Institute, The University of Sydney, Sydney, NSW, Australia

  2. 2

    Discipline of Anatomy & Histology, School of Medical Sciences, The University of Sydney, Sydney, NSW, Australia

  3. 3

    School of Optometry & Vision Science, University of NSW, Sydney, NSW, Australia

  4. 4

    ARC Centre of Excellence in Vision Science, ANU Medical School, and Research School of Biological Sciences, Australian National University, Canberra, ACT, Australia

*Dr Michele C. Madigan, Phd Save Sight Institute GPO Box 4337 Sydney, NSW 2001 Australia Tel: 61 0 2 9382 7283 Fax: 61 2 9382 7318 Email: michele@eye.usyd.edu.au

Publication History

  1. Issue published online: 18 APR 2011
  2. Article first published online: 29 JUL 2010
  3. Received on March 11th, 2010. Accepted on May 28th, 2010.

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Keywords:

  • endothelial cells;
  • fibroblast growth factor;
  • foveal avascular zone;
  • Müller cells;
  • primate retina;
  • transforming growth factor-β

Abstract.

Purpose:  During retinal development, the pattern of blood vessel formation depends upon the combined effects of proliferation and migration of endothelial cells, astrocytes and Müller cells. In this study, we investigated the potential for transforming growth factor-β (TGF-β) and fibroblast growth factor (FGF-2) to influence this process by regulating proliferation and migration of retinal endothelial and macroglial cells.

Methods:  We assessed the effects of exogenous TGF-β and FGF-2 on the proliferation and migration of cultured endothelial (RF/6A) and Müller cell (MIO-M1) lines. Cell proliferation was measured using a MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) colorimetric assay over 72 hr. Cell migration was measured using a scratch-wound assay over 72 hr.

Results:  Transforming growth factor-β inhibited the proliferation of endothelial and Müller cells and inhibited the migration of Müller cells, but not endothelial cells, compared to untreated controls. Conversely, FGF-2 increased endothelial cell proliferation but inhibited endothelial cell migration. Fibroblast growth factor-2 increased migration of Müller cells but had little effect on proliferation except at higher concentrations (20 ng/ml).

Conclusion:  Taken together, these observations indicate that TGF-β and FGF could work in concert to inhibit endothelial cell proliferation and migration, respectively; this may have implications for establishing and maintaining the avascular zone of primate fovea.

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