Retinal vessel diameter can reliably be determined in minipigs using Retinal Vessel Analyser with a microscope-mounted fundus camera
Article first published online: 9 MAR 2012
© 2012 The Authors. Acta Ophthalmologica © 2012 Acta Ophthalmologica Scandinavica Foundation
Volume 90, Issue 4, pages e269–e273, June 2012
How to Cite
Papadopoulou, D. N., Mangioris, G., Petropoulos, I. K., Mendrinos, E., Mavropoulos, A. and Pournaras, C. J. (2012), Retinal vessel diameter can reliably be determined in minipigs using Retinal Vessel Analyser with a microscope-mounted fundus camera. Acta Ophthalmologica, 90: e269–e273. doi: 10.1111/j.1755-3768.2011.02374.x
- Issue published online: 28 MAY 2012
- Article first published online: 9 MAR 2012
- Received on July 10th, 2011. Accepted on December 6th, 2011.
- fundus camera;
- Retinal Vessel Analyser;
- retinal vessel diameter
Purpose: Retinal Vessel Analyser (RVA) is a validated instrument to measure retinal vessel diameter in humans. The purpose of this study was to assess the reproducibility (inter-observer reliability) and the repeatability (test–retest reliability) of RVA with a microscope-mounted fundus camera to determine retinal vessel diameter in minipigs.
Methods: Ocular fundus image from five anaesthetized minipigs was recorded in a digital videotape for approximately 5 min, under stable systemic arterial pressure and gas conditions. To evaluate the reproducibility, each one of two investigators used RVA to measure the diameter of the superior temporal retinal artery on five separate 30-second video sequences from each minipig, which were the same video sequences for both investigators. To evaluate the repeatability, one investigator performed five measurements on a single, randomly selected, 30-second video sequence from each minipig. The reproducibility was determined using the intra-class correlation coefficient (ICC), and the repeatability was assessed using the coefficient of variation (COV). Bland–Altman plots were also used to assess agreement between the two investigators.
Results: Retinal arteriolar diameter measurements with RVA in minipigs were highly reproducible. Differences between the two investigators were lower than 0.7%. The ICC was 1.00, indicating perfect reproducibility, and the mean COV was 0.18%, reflecting excellent repeatability of the measurements with RVA.
Conclusion: Retinal vessel diameter can reliably be determined not only in humans, but also in minipigs, using the commercially available RVA apparatus and a microscope-mounted fundus camera.