Cytokine concentration in aqueous humour of eyes with exudative age-related macular degeneration
Article first published online: 10 APR 2012
© 2012 The Authors. Acta Ophthalmologica © 2012 Acta Ophthalmologica Scandinavica Foundation
Volume 90, Issue 5, pages e381–e388, August 2012
How to Cite
Jonas, J. B., Tao, Y., Neumaier, M. and Findeisen, P. (2012), Cytokine concentration in aqueous humour of eyes with exudative age-related macular degeneration. Acta Ophthalmologica, 90: e381–e388. doi: 10.1111/j.1755-3768.2012.02414.x
- Issue published online: 27 JUL 2012
- Article first published online: 10 APR 2012
- Received on May 13th, 2011. Accepted on January 25th, 2012.
- epithelial growth factor;
- exudative age-related macular degeneration;
- intercellular adhesion molecule-1;
- monocyte chemoattractant protein-1
Purpose: To measure the concentration of cytokines in the aqueous humour of eyes with exudative age-related macular degeneration (AMD).
Methods: The clinical interventional study included a study group of 18 patients with exudative AMD and a control group of 20 patients undergoing routine cataract surgery. Age did not vary significantly (p = 0.36) between study group (80.8 ± 6.4 years) and control group (77.0 ± 9.9 years), nor did gender (p = 0.75). During the interventions, aqueous humour samples were obtained, in which the concentration of cytokines was measured using a solid-phase chemiluminescence immunoassay. Macular thickness was measured by optical coherence tomography (OCT).
Results: In the study group as compared to the control group, significantly higher concentrations were measured for epithelial growth factor (EGF) (p = 0.017), human growth factor (HGF) (p = 0.048), intercellular adhesion molecule-1 (ICAM1) (p = 0.028), interleukin 12p40 (IL12p40) (p = 0.009), interleukin 1a2 (IL1a2) (p = 0.01), interleukin 3 (IL3) (p = 0.02), interleukin 6 (IL6) (p = 0.006), interleukin 8 (IL8) (p = 0.02), monocyte chemoattractant protein-1 (MCP-1) (p = 0.048), monokine induced by interferon gamma (MIG) (p = 0.016), matrix metalloproteinase 9 (MMP9) (p = 0.004) and plasminogen activator inhibitor 1 (PAI1) (p = 0.006). Macular thickness was significantly associated with the concentrations of EGF (p = 0.001), HGF (p = 0.02), ICAM1 (p = 0.001), interleukin 12p40 (p = 0.006), IL 1a2 (p = 0.002), MIG (p = 0.001), MMP9 (p < 0.001) and PAI1 (p = 0.01). Interleukin 6 and MCP-1 showed significant associations with the height of retinal pigment epithelium detachment.
Conclusions: Numerous cytokines are associated with the presence and the amount of exudative AMD.