• Open Access

Enzymatic deconstruction of xylan for biofuel production

Authors

  • DYLAN DODD,

    1. Department of Microbiology, University of Illinois, Urbana, IL 61801, USA,
    2. Energy Biosciences Institute, University of Illinois, Urbana, IL 61801, USA,
    3. Institute for Genomic Biology, University of Illinois, Urbana, IL 61801, USA,
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  • ISAAC K. O. CANN

    1. Department of Microbiology, University of Illinois, Urbana, IL 61801, USA,
    2. Energy Biosciences Institute, University of Illinois, Urbana, IL 61801, USA,
    3. Institute for Genomic Biology, University of Illinois, Urbana, IL 61801, USA,
    4. Department of Animal Sciences, University of Illinois, Urbana, IL 61801, USA
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Isaac K. O. Cann, tel. +1 217 333 2090, fax +1 217 333 8286, e-mail: icann@illinois.edu

Abstract

The combustion of fossil-derived fuels has a significant impact on atmospheric carbon dioxide (CO2) levels and correspondingly is an important contributor to anthropogenic global climate change. Plants have evolved photosynthetic mechanisms in which solar energy is used to fix CO2 into carbohydrates. Thus, combustion of biofuels, derived from plant biomass, can be considered a potentially carbon neutral process. One of the major limitations for efficient conversion of plant biomass to biofuels is the recalcitrant nature of the plant cell wall, which is composed mostly of lignocellulosic materials (lignin, cellulose, and hemicellulose). The heteropolymer xylan represents the most abundant hemicellulosic polysaccharide and is composed primarily of xylose, arabinose, and glucuronic acid. Microbes have evolved a plethora of enzymatic strategies for hydrolyzing xylan into its constituent sugars for subsequent fermentation to biofuels. Therefore, microorganisms are considered an important source of biocatalysts in the emerging biofuel industry. To produce an optimized enzymatic cocktail for xylan deconstruction, it will be valuable to gain insight at the molecular level of the chemical linkages and the mechanisms by which these enzymes recognize their substrates and catalyze their reactions. Recent advances in genomics, proteomics, and structural biology have revolutionized our understanding of the microbial xylanolytic enzymes. This review focuses on current understanding of the molecular basis for substrate specificity and catalysis by enzymes involved in xylan deconstruction.

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