PKS and NRPS gene clusters from microbial symbiont cells of marine sponges by whole genome amplification
Article first published online: 31 JUL 2009
© 2009 Society for Applied Microbiology and Blackwell Publishing Ltd
Environmental Microbiology Reports
Special Issue: Symbiosis. Editors: Professors Paola Bonfante, Karen Visick, and Moriya Ohkuma
Volume 2, Issue 4, pages 507–513, August 2010
How to Cite
Siegl, A. and Hentschel, U. (2010), PKS and NRPS gene clusters from microbial symbiont cells of marine sponges by whole genome amplification. Environmental Microbiology Reports, 2: 507–513. doi: 10.1111/j.1758-2229.2009.00057.x
- Issue published online: 16 JUL 2010
- Article first published online: 31 JUL 2009
- Received 22 December, 2008; accepted 16 June, 2009.
Whole genome amplification (WGA) approaches provide genomic information on single microbial cells and hold great promise for the field of environmental microbiology. Here, the microbial consortia of the marine sponge Aplysina aerophoba were sorted by fluorescence-activated cell sorting (FACS) and then subjected to WGA. A cosmid library was constructed from the WGA product of a sample containing two bacterial cells, one a member of the candidate phylum Poribacteria and one of a sponge-specific clade of Chloroflexi. Library screening led to the genomic characterization of three cosmid clones, encoding a polyketide synthase (PKS), a non-ribosomal peptide synthetase (NRPS) and the Chloroflexi 16S rRNA gene. PCR screening of WGA products from additional, FACS-sorted single bacterial symbiont cells supports the assignment of the Sup-PKS gene to the Poribacteria and the novel NRPS gene to the Chloroflexi. This promising single-cell genomics approach has permitted cloning of entire gene clusters from single microbial cells of known phylogenetic origin and thus provides a sought-after link between phylogeny and function.