Substantial high-affinity methanotroph populations in Andisols effect high rates of atmospheric methane oxidation
Article first published online: 27 AUG 2009
© 2009 Society for Applied Microbiology and Blackwell Publishing Ltd
Environmental Microbiology Reports
Special Issue: Methane Cycle. Editors: Professor J. Colin Murrell and Professor Mike S. M. Jetten
Volume 1, Issue 5, pages 450–456, October 2009
How to Cite
Maxfield, P. J., Hornibrook, E. R.C. and Evershed, R. P. (2009), Substantial high-affinity methanotroph populations in Andisols effect high rates of atmospheric methane oxidation. Environmental Microbiology Reports, 1: 450–456. doi: 10.1111/j.1758-2229.2009.00071.x
- Issue published online: 8 OCT 2009
- Article first published online: 27 AUG 2009
- Received 15 April, 2009; accepted 20 July, 2009.
Methanotrophic bacteria in soils derived from volcanic ash (Andisols) were characterized via time series 13C-phospholipid fatty acid (PLFA) labelling. Three Andisols were incubated under 2 ppmv 13CH4 for up to 18 weeks, thus enabling high-affinity methanotrophs to be selectively characterized and quantified. PLFA profiles from all soils were broadly similar, but the magnitude of the high-affinity methanotrophic populations determined through 13C-PLFA-stable isotope probing displayed sizeable differences. Substantial incorporation of 13C indicated very large high-affinity methanotrophic populations in two of the soils. Such high values are far in excess (10×) of those observed for a range of mineral soils incubated under similar conditions (Bull et al., 2000; Maxfield et al., 2006; 2008a, b). Two of the three Andisols studied also displayed high but variable CH4 oxidation rates ranging from 0.03 to 1.58 nmol CH4 g−1 d.wt. h−1. These findings suggest that Andisols, a previously unstudied soil class with respect to high-affinity methanotrophic bacteria, may oxidize significant amounts of atmospheric methane despite their low areal coverage globally.