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Fig. S1. Stable isotope enrichment in the methane pool during anaerobic oxidation of methane with sulfate in sediment-free enrichment cultures from Amon Mud Volcano sediment and Black Sea mat.

Table S1. Origin of samples.

Table S2. Medium, incubation and sampling.

Table S3. Analytical methods.

Table S4. Phylogenetic assignment and quantification of microorganisms.

Appendix S1. Calculation of possible effect of mass transfer on isotope fractionation.

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