Chlorophyll a might structure a community of potentially pathogenic culturable Vibrionaceae. Insights from a one-year study of water and mussels surveyed on the French Atlantic coast

Authors

  • J. Deter,

    1. Ifremer, centre de Brest, Département Environnement, Microbiologie et Phycotoxines (EMP), Laboratoire de Microbiologie, ZI de la pointe du diable, B.P. 70, 29280 Plouzané, France.
    Search for more papers by this author
  • S. Lozach,

    1. Ifremer, centre de Brest, Département Environnement, Microbiologie et Phycotoxines (EMP), Laboratoire de Microbiologie, ZI de la pointe du diable, B.P. 70, 29280 Plouzané, France.
    Search for more papers by this author
  • A. Derrien,

    1. Ifremer, LERPC, centre de La Rochelle, place Gaby Coll, BP 7, 17137 L'Houmeau, France.
    Search for more papers by this author
  • A. Véron,

    1. Ifremer, centre de Nantes, EMP/Laboratoire National de Référence Microbiologie des Coquillages, BP 21105, 44311 Nantes Cedex 3, France.
    Search for more papers by this author
  • J. Chollet,

    1. Ifremer, centre de Brest, Département Environnement, Microbiologie et Phycotoxines (EMP), Laboratoire de Microbiologie, ZI de la pointe du diable, B.P. 70, 29280 Plouzané, France.
    Search for more papers by this author
  • D. Hervio-Heath

    Corresponding author
    1. Ifremer, centre de Brest, Département Environnement, Microbiologie et Phycotoxines (EMP), Laboratoire de Microbiologie, ZI de la pointe du diable, B.P. 70, 29280 Plouzané, France.
    Search for more papers by this author

E-mail Dominique.Hervio.Heath@ifremer.fr; Tel. (+33) 2 98 22 43 49; Fax (+33) 2 98 22 45 94.

Summary

The present study focused on the isolation of culturable bacteria from mussels and sea water to identify Vibrionaceae potentially pathogenic for humans. Three sites located on the French Atlantic coast were monitored monthly (twice each month during summer) for 1 year. Environmental parameters were surveyed (water temperature, salinity, turbidity, chlorophyll a) and bacteria were detected by culture and identified by API 20E® systems (BioMérieux) and PCR. A total of seven species were detected (Grimontia hollisae, Photobacterium damselae, Vibrio alginolyticus, V. cholerae, V. fluvialis, V. vulnificus and V. parahaemolyticus) and species diversity was higher at the end of summer. Surprisingly, V. cholerae non-O1/non-O139 was detected in spring. No site effect was detected. Using Sørensen similarity indices and statistical analyses, we showed that chlorophyll a had a significant influence on the bacterial community detected in mussels and assemblages were more similar to one another when chlorophyll a values were above 20 µg l−1. No significant effect of any parameter was found on the community detected in water samples. Such surveys are essential for the understanding of sanitary crises and detection of emerging pathogens.

Ancillary