Fig. S1. (A) Monthly composite of sea surface chlorophyll for the three ocean basins sampled during the Blue Earth Global Expedition (BEAGLE) 2003. The red dots denote the location of the sampling stations. Also shown are the boundaries of the biogeochemical provinces as described in Longhurst (1998): AUSE, East Australian Coastal Province; ARCH, Archipelagic Deep Basins Province; SPSG, South Pacific Subtropical Gyre Province; HUMB, Humboldt Current Coastal Province; BRAZ, Brazil Current Coastal Province; SATL, South Atlantic Gyral Province; BENG, Benguela Current Coastal Province; EAFR, Eastern Africa Coastal Province; ISSG, Indian South Subtropical Gyre Province, AUSW, Australia-Indonesia Coastal Province (B) Longitudinal variability in the relative contribution of accessory pigments to total accessory pigment concentration along the BEAGLE transect. Photoprotective pigments include α and β carotene, diatoxanthin and diadinoxanthin (Diat + Diad) and zeaxanthin (Zeax). Photosynthetic pigments include chlorophyll b (Chl b), 19′-hexanoyloxyfucoxanthin (19′-Hex), 19′-butanoyloxyfucoxanthin (19′-But), chlorophyll c3 (Chl c3), fucoxanthin (Fucox) and chlorophyll c1+2 (Chl c1+2).

Fig. S2. Relative hybridization signal (as a proportion of all products amplified using the OXY107F-OXY1313R primer pair; see Fuller et al., 2003) for the (A) four Prochlorococcus ecotypes and (B) 10 clades of marine Synechococcus across the BEAGLE transect. 16S rRNA gene amplicons from environmental DNA and from control strains were purified, blotted onto nylon membranes and hybridized to oligonucleotide probes specific for different Prochlorococcus ecotypes and Synechococcus lineages following the method of Fuller and colleagues (2003), and MC-A clade X which was recently reassigned to a new sub-cluster, 5.3 (Dufresne et al., 2008). The Synechococcus probes specifically target lineages I to X within the MC-A (sub-cluster 5.1) taxon (Fuller et al., 2003), which contains the most abundant representatives in the open-ocean and coastal environments, and together with Prochlorococcus forms a discrete picophytoplankton clade (Urbach et al., 1998). The oligonucleotide probe sequences used in this study have been previously published (Fuller et al., 2003). Using a Fujifilm FLA-5000 phosphorimager hybridization signals were quantified. Relative hybridization (%) was calculated as previously described (Fuller et al., 2003; Bouman et al., 2006).

Table S1. Distribution of pigment markers among algal taxa used in this study. Algal groups in bold are considered to be the groups most commonly ascribed to the indicator pigment.

Appendix S1. Supplementary experimental procedures.

EMI4_241_sm_AppendixS1.doc34KSupporting info item
EMI4_241_sm_FigureS1-S2_legend.doc30KSupporting info item
EMI4_241_sm_FigureS1.tif433KSupporting info item
EMI4_241_sm_FigureS2.tif105KSupporting info item
EMI4_241_sm_TableS1.doc29KSupporting info item

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