Chitin stimulates production of the antibiotic andrimid in a Vibrio coralliilyticus strain
Article first published online: 9 MAY 2011
© 2011 Society for Applied Microbiology and Blackwell Publishing Ltd
Environmental Microbiology Reports
Volume 3, Issue 5, pages 559–564, October 2011
How to Cite
Wietz, M., Månsson, M. and Gram, L. (2011), Chitin stimulates production of the antibiotic andrimid in a Vibrio coralliilyticus strain. Environmental Microbiology Reports, 3: 559–564. doi: 10.1111/j.1758-2229.2011.00259.x
- Issue published online: 2 OCT 2011
- Article first published online: 9 MAY 2011
- Received 30 November, 2010; accepted 30 March, 2011.
Vibrio coralliilyticus is a putative coral pathogen in tropical oceans, but also possesses antagonistic traits. We previously reported antibacterial activity in Vibrio coralliilyticus strain S2052 based upon the antibiotic andrimid. The purpose of the present study was to determine whether V. coralliilyticus S2052 produces the antibiotic under conditions mimicking natural habitats of vibrios. S2052 synthesized andrimid with both chitin and macroalgal extracts as sole nutrient source. With chitin, the biosynthesis of metabolites other than andrimid was largely abolished, and the yield of the antibiotic per cell was twofold higher. In cultures with Artemia as live chitin model system, S2052 reached up to 108 cells ml−1, produced andrimid and showed attachment to the exoskeleton and chitinous exuviae. The metabolic focus on andrimid production with chitin indicates that the antibiotic could serve an ecophysiological function. S2052 was compared with two related V. coralliilyticus strains (LMG20984T and LMG10953). Despite overall similar secondary metabolomes, LMG20984T and LMG10953 did not produce andrimid, and their optimum biosynthetic temperature was 30 as compared with 25°C for S2052. In addition, S2052 appeared less pathogenic towards Artemia than reported for the type strain. Different physiologies of S2052 and closely related strains indicated that V. coralliilyticus subspecies may be adapted to different niches.