Present address: Centre de Recherche Public – Gabriel Lippmann, and Luxembourg Center for Systems Biomedicine, University of Luxembourg, Belvaux, Grand-Duchy of Luxembourg.
Correlative microscopy for phylogenetic and ultrastructural characterization of microbial communities
Article first published online: 22 AUG 2011
© 2011 Society for Applied Microbiology and Blackwell Publishing Ltd
Environmental Microbiology Reports
Thematic issue: Taxonomy and Biodiversity
Volume 4, Issue 1, pages 36–41, February 2012
How to Cite
Knierim, B., Luef, B., Wilmes, P., Webb, R. I., Auer, M., Comolli, L. R. and Banfield, J. F. (2012), Correlative microscopy for phylogenetic and ultrastructural characterization of microbial communities. Environmental Microbiology Reports, 4: 36–41. doi: 10.1111/j.1758-2229.2011.00275.x
Author contributions: B.K., B.L., P.W., R.I.W., M.A., L.R.C. and J.F.B. designed the research; B.K., B.L., P.W. and R.I.W. developed and performed the CARD-FISH; B.L. and L.R.C. cultured Caulobacter crescentus and B.L. prepared AMD biofilm samples; B.K., P.W. and R.I.W. prepared the resin samples and B.K. carried out the resin section transmission electron microscopy; B.L. and L.R.C. prepared the cryogenic samples and did the cryogenic electron microscopy; B.L. performed the electron dose calibration and optimization; B.K., B.L. and P.W. performed the fluorescence microscopy and confocal laser scanning microscopy; B.K., B.L. and L.R.C. prepared the figures; B.K., B.L., L.R.C. and J.F.B. prepared the manuscript. All authors contributed to the manuscript.
- Issue published online: 7 FEB 2012
- Article first published online: 22 AUG 2011
- Received 13 April, 2011; accepted 18 June, 2011.
Transmission electron microscopy (TEM) can provide ultrastructural information for cells in microbial community samples and phylogenetic information can be recovered via molecular surveys. Here we report an approach to link these data sets by coupling fluorescence in situ hybridization (FISH) with either conventional biological or cryogenic TEM. The method could fundamentally improve our understanding of the organization and functioning of microbial communities in natural systems.