The temporal dynamics of bacterial communities across human anterior nares
Article first published online: 12 DEC 2011
© 2011 Society for Applied Microbiology and Blackwell Publishing Ltd
Environmental Microbiology Reports
Thematic issue: Taxonomy and Biodiversity
Volume 4, Issue 1, pages 126–132, February 2012
How to Cite
Camarinha-Silva, A., Jáuregui, R., Pieper, D. H. and Wos-Oxley, M. L. (2012), The temporal dynamics of bacterial communities across human anterior nares. Environmental Microbiology Reports, 4: 126–132. doi: 10.1111/j.1758-2229.2011.00313.x
- Issue published online: 7 FEB 2012
- Article first published online: 12 DEC 2011
- Received 28 September, 2011; revised 11 November, 2011; accepted 13 November, 2011.
Fig. S1. Non-metric multidimensional scaling (nMDS) plot illustrating similarities in the global bacterial community structure of the anterior nares of 25 volunteers during 15 months using T-RFLP data collected from 16S rRNA gene amplicons digested consecutively with AseI, TspRI and ApekI as previously described (Camarinha-Silva et al., 2011). The Bray–Curtis similarity algorithm (Bray and Curtis, 1957) and nMDS algorithm using 50 random restarts (Clarke, 1993) was used to construct the ordination plot. While a 2D stress value of 0.2 indicates some stress on the plot, it is deemed acceptable considering that 363 samples are being ordinated together. Numbers 1–25 correspond to individual volunteers.
Table S1. Volunteer details.
Table S2. Environmental data for the Lower-Saxony region (average minimum and maximum temperatures, average hours of sunlight and average maximum daily precipitation) at each month over the nasal sampling time frame (November 2009 to January 2011). These data were collected from the National German meteorology website Deutscher Wetterdienst (http://www.dwd.de).
Table S3. Pollen dispersion data for the Lower-Saxony region at each month over the nasal sampling time frame (November 2009 to January 2011). These data were collected from the National German meteorology website Deutscher Wetterdienst (http://www.dwd.de).
Table S4. Relative abundance (%) of the species depicted in Fig. 3. Only on 12 occasions could a sample not be collected from a volunteer (as indicated in the table by -). Months A–O respectively represent the months from November 2009 to January 2011. Prevalence (%) across the time points and carriage status are also given. Carriage status: No-colonization (0% prevalence over time); Transient (1–9% prevalence over time); Intermittent (10–44% prevalence over time); Persistent (45–89% prevalence over time) and Permanent (> 90% prevalence over time).
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