Type III effector genes and other virulence factors of Shiga toxin-encoding Escherichia coli isolated from wastewater
Article first published online: 21 DEC 2011
© 2011 Society for Applied Microbiology and Blackwell Publishing Ltd
Environmental Microbiology Reports
Thematic issue: Taxonomy and Biodiversity
Volume 4, Issue 1, pages 147–155, February 2012
How to Cite
Martínez-Castillo, A., Allué-Guardia, A., Dahbi, G., Blanco, J., Creuzburg, K., Schmidt, H. and Muniesa, M. (2012), Type III effector genes and other virulence factors of Shiga toxin-encoding Escherichia coli isolated from wastewater. Environmental Microbiology Reports, 4: 147–155. doi: 10.1111/j.1758-2229.2011.00317.x
- Issue published online: 7 FEB 2012
- Article first published online: 21 DEC 2011
- Received 9 May, 2011; accepted 22 November, 2011.
Fig. S1. Number of virulence genes found in each serotype in the isolates showing positive results for one or more genes. (A) All positive serotypes; (B) positive serotypes distributed by wastewater origin.
Table S1. Effector genes analysed in this study.
Table S2. Serotypes comprising the environmental collection. Serotyping of O and H antigens of the strains were determined according to the method described by Guinée and colleagues (1981) employing all available O (O1 to O181) and H (H1 to H56) antisera. All antisera were obtained and absorbed with the corresponding cross-reacting antigens to remove non-specific agglutinins.
Table S3. Oligonucleotides, target genes, binding sites and product size for detection of all genes analysed in this study. PCRs were performed with a GeneAmp PCR system 2400 (Perkin-Elmer, PE Applied Biosystems, Barcelona, Spain) and using the primers described in Table S3. The conditions for amplification were 1 cycle at 94°C and 5 min for denaturation, followed by 35 cycles at 94°C for 1.5 min, 1 min at the annealing temperature corresponding to each oligonucleotide and elongation at 72°C according to the product size (1 min for each 1000 bp). Finally, 1 cycle at 72°C for 4 min ended the amplification procedure. The PCR products were analysed by gel electrophoresis on a 1% agarose gel and bands were visualized by ethidium bromide staining. For sequencing the same primers were used.
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