SEARCH

SEARCH BY CITATION

Fig. S1. Number of virulence genes found in each serotype in the isolates showing positive results for one or more genes. (A) All positive serotypes; (B) positive serotypes distributed by wastewater origin.

Table S1. Effector genes analysed in this study.

Table S2. Serotypes comprising the environmental collection. Serotyping of O and H antigens of the strains were determined according to the method described by Guinée and colleagues (1981) employing all available O (O1 to O181) and H (H1 to H56) antisera. All antisera were obtained and absorbed with the corresponding cross-reacting antigens to remove non-specific agglutinins.

Table S3. Oligonucleotides, target genes, binding sites and product size for detection of all genes analysed in this study. PCRs were performed with a GeneAmp PCR system 2400 (Perkin-Elmer, PE Applied Biosystems, Barcelona, Spain) and using the primers described in Table S3. The conditions for amplification were 1 cycle at 94°C and 5 min for denaturation, followed by 35 cycles at 94°C for 1.5 min, 1 min at the annealing temperature corresponding to each oligonucleotide and elongation at 72°C according to the product size (1 min for each 1000 bp). Finally, 1 cycle at 72°C for 4 min ended the amplification procedure. The PCR products were analysed by gel electrophoresis on a 1% agarose gel and bands were visualized by ethidium bromide staining. For sequencing the same primers were used.

FilenameFormatSizeDescription
EMI4_317_sm_FigS1-TabS1-3.doc229KSupporting info item

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.