An ATP-driven potassium pump promotes long-term survival of Halobacterium salinarum within salt crystals
Article first published online: 10 FEB 2012
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Environmental Microbiology Reports
Volume 4, Issue 2, pages 234–241, April 2012
How to Cite
Kixmüller, D. and Greie, J.-C. (2012), An ATP-driven potassium pump promotes long-term survival of Halobacterium salinarum within salt crystals. Environmental Microbiology Reports, 4: 234–241. doi: 10.1111/j.1758-2229.2012.00326.x
- Issue published online: 15 MAR 2012
- Article first published online: 10 FEB 2012
- Received 15 August, 2011; revised 22 December, 2011; accepted 6 January, 2012.
Many extremely halophilic archaea belonging to the Halobacteriales have remarkable longevity. They are even known to persist for millions of years within fluid inclusions of salt crystals. However, the key systems responsible for this remarkable ability and the underlying physiological mechanisms have not yet been deciphered. This study revealed that the ATP-dependent K+ uptake system KdpFABC of Halobacterium salinarum is essential for survival under desiccation and salt crystal inclusion and, thus, can be identified as at least one of these systems in this organism. The presence of the kdp genes promoted survival of H. salinarum entombed in halite, compared with cells in which these genes were deleted. Expression of the kdp operon was found to be induced already under desiccating conditions without halite entombment. The morphology of cells included in halite resembled that of cells grown under potassium limitation. Therefore, a steady potassium supply, even under unfavourable energetic conditions, plays a key role in long-term survival and desiccation tolerance.