An ATP-driven potassium pump promotes long-term survival of Halobacterium salinarum within salt crystals
Version of Record online: 10 FEB 2012
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Environmental Microbiology Reports
Volume 4, Issue 2, pages 234–241, April 2012
How to Cite
Kixmüller, D. and Greie, J.-C. (2012), An ATP-driven potassium pump promotes long-term survival of Halobacterium salinarum within salt crystals. Environmental Microbiology Reports, 4: 234–241. doi: 10.1111/j.1758-2229.2012.00326.x
- Issue online: 15 MAR 2012
- Version of Record online: 10 FEB 2012
- Received 15 August, 2011; revised 22 December, 2011; accepted 6 January, 2012.
Fig. S1. β-Galactosidase (bgaH) activity of H. salinarum R1 (WT) and H. salinarum ΔkdpFABC with plasmid-encoded bgaH fused to either functional kdp promoter (−206) or to non-functional truncated kdp promoter (−25) measured by the ONPG assay described by Holmes et al., 1997. Liquid cultures were grown in either rich medium (ATCC 2185) and in casamino acids medium (CAA) supplemented with KCl as indicated as described by Strahl and Greie (2008). Error bars correspond to the mean standard deviation of three independent measurements.
Movie S1. Z-scan of a halite inclusion with entrapped H. salinarum R1 cells. Scale bar: 10 µM. Photomicrographs were acquired with a Leica DM5500B microscope equipped with a SPOT Pursuit-XS camera.
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