Plant lectin-like antibacterial proteins from phytopathogens Pseudomonas syringae and Xanthomonas citri
Article first published online: 20 FEB 2012
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Environmental Microbiology Reports
Special Issue: Ecology, Evolution and Population Genetics of Pathogenic Microbes
Volume 4, Issue 4, pages 373–380, August 2012
How to Cite
Ghequire, M. G. K., Li, W., Proost, P., Loris, R. and De Mot, R. (2012), Plant lectin-like antibacterial proteins from phytopathogens Pseudomonas syringae and Xanthomonas citri. Environmental Microbiology Reports, 4: 373–380. doi: 10.1111/j.1758-2229.2012.00331.x
- Issue published online: 16 JUL 2012
- Article first published online: 20 FEB 2012
- Received 17 November, 2011; revised 15 January, 2012; accepted 30 January, 2012.
The genomes of Pseudomonas syringae pv. syringae 642 and Xanthomonas citri pv. malvacearum LMG 761 each carry a putative homologue of the plant lectin-like bacteriocin (llpA) genes previously identified in the rhizosphere isolate Pseudomonas putida BW11M1 and the biocontrol strain Pseudomonas fluorescens Pf-5. The respective purified recombinant proteins, LlpAPss642 and LlpAXcm761, display genus-specific antibacterial activity across species boundaries. The inhibitory spectrum of the P. syringae bacteriocin overlaps partially with those of the P. putida and P. fluorescens LlpAs. Notably, Xanthomonas axonopodis pv. citri str. 306 secretes a protein identical to LlpAXcm761. The functional characterization of LlpA proteins from two different phytopathogenic γ-proteobacterial species expands the lectin-like bacteriocin family beyond the Pseudomonas genus and suggests its involvement in competition among closely related plant-associated bacteria with different lifestyles.