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Fig. S1. Principal component analyses of Archaea 16S rRNA, mcrA, dsrA and Desulfovibrio 16S rRNA gene sequences. A nested PCR-TRFLP approach was used for the amplification of Archaea 16S rRNA, mcrA, dsrA and Desulfovibrio spp. 16S rRNA genes from stool samples. Sample preparation and data analyses were executed as described in Fig. 1. Differences in the structure and composition of Archaea 16S rRNA, mcrA, dsrA and Desulfovibrio spp. 16S rRNA gene sequences between the three groups were examined by multivariate analysis. Principal component analysis of (A) Archaea 16S rRNA gene TRF, (B) mcrA TRF, (C) dsrA TRF and (D) Desulfovibrio spp. 16S rRNA gene TRF. Green symbols represent NA; blue symbols, AA and red symbols, EA. Each symbol designates a single subject. Samples are plotted along the first two principal component axes. The ellipses of PCA correspond to the joint 95% confidence limits.

Table S1. List of Primers and Annealing Temperatures Used in This Study.

Appendix S1. Study populations and statistics.

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EMI4_334_sm_FS1.pdf63KSupporting info item
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