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Fig. S1. The transcript abundance of IPF-469. (A) In early-log phase cultures of Microcystis sp. strain GK normalized to the level observed in stationary phase cultures. Both treatments were normalized to untreated controls in Microcystis sp. strain GK exposed to spent medium from Scenedesmus sp. strain HUJI (S. HUJI) or to its own cell-debris (‘cell-extract’). In late log phase mutant ΔmcyB normalized against its WT, M. aeruginosa PCC 7806. No significant difference was observed between the treatments (B). M. aeruginosa PCC 7806 grown under light/dark cycles, the data were re-generated from Straub and colleagues (2011).

Fig. S2. Neighbour-joining phylogenetic tree of (A) IPF-5130 and (B) IPF-5227 homologues; accession numbers are provided in brackets.

Table S1. A list of Microcystis strains examined in this study.

Table S2. Amino acid composition (percentage of the total) in purified fibres isolated from Microcystis sp. strain GK.

Table S3. A summary of the isolated fibrous associated proteins.

Table S4. The primers used in this study for the qRT-PCR procedure.

Appendix S1. Supplementary experimental procedures.

FilenameFormatSizeDescription
EMI4_339_sm_FigureS1.tif145KSupporting info item
EMI4_339_sm_FigureS2.tif1143KSupporting info item
EMI4_339_sm_TableS1-S4_AppendixS1.doc83KSupporting info item

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