Casting a net: fibres produced by Microcystis sp. in field and laboratory populations
Article first published online: 29 MAR 2012
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Environmental Microbiology Reports
Volume 4, Issue 3, pages 342–349, June 2012
How to Cite
Harel, M., Weiss, G., Daniel, E., Wilenz, A., Hadas, O., Sukenik, A., Sedmak, B., Dittmann, E., Braun, S. and Kaplan, A. (2012), Casting a net: fibres produced by Microcystis sp. in field and laboratory populations. Environmental Microbiology Reports, 4: 342–349. doi: 10.1111/j.1758-2229.2012.00339.x
- Issue published online: 10 MAY 2012
- Article first published online: 29 MAR 2012
- Received 13 December, 2011; accepted 2 March, 2012.
Fig. S1. The transcript abundance of IPF-469. (A) In early-log phase cultures of Microcystis sp. strain GK normalized to the level observed in stationary phase cultures. Both treatments were normalized to untreated controls in Microcystis sp. strain GK exposed to spent medium from Scenedesmus sp. strain HUJI (S. HUJI) or to its own cell-debris (‘cell-extract’). In late log phase mutant ΔmcyB normalized against its WT, M. aeruginosa PCC 7806. No significant difference was observed between the treatments (B). M. aeruginosa PCC 7806 grown under light/dark cycles, the data were re-generated from Straub and colleagues (2011).
Fig. S2. Neighbour-joining phylogenetic tree of (A) IPF-5130 and (B) IPF-5227 homologues; accession numbers are provided in brackets.
Table S1. A list of Microcystis strains examined in this study.
Table S2. Amino acid composition (percentage of the total) in purified fibres isolated from Microcystis sp. strain GK.
Table S3. A summary of the isolated fibrous associated proteins.
Table S4. The primers used in this study for the qRT-PCR procedure.
Appendix S1. Supplementary experimental procedures.
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