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Supplementary experimental procedures.

Supplementary results.

Fig. S1. Analysis of α-diversity in relation to sequencing depth. (A, D, G) Shannon index; (B, E, H) Chao1 richness; (C, F, I) Pielou's evenness; (A, B, C) 15 lakes (‘Water07’); (D, E, F) 10 sediment samples (‘Sediments’) and (G, H, I) 5 lakes and 1 stream (‘Water10’).

Fig. S2. Analysis of β-diversity. (A) Bray-Curtis values of 15 lakes in relation to sequencing depth (‘Water07’); (B) DCA plot of the Water07 samples using 20 000 sequences per sample; (C) Bray-Curtis values of 10 sediment samples in relation to sequencing depth (‘Sediments’); (D) DCA plot of the sediment samples using 15 000 sequences per sample; (E) Bray-Curtis values of five lakes and 1 stream in relation to sequencing depth (‘Water10’); (F) DCA plot of the Water10 samples using 15 000 sequences per sample. Abbreviations in DCA plots are the same as in Table S1.

Fig. S3. Design of the study.

Table S1. Physicochemical and biological characteristics of the study systems.

Table S2. The loss of sequences due to the perseus Chimera check step. # sequences denotes the number of sequences in each sample before chimera check. Within each data set and sequencing depth each sample was resampled to the minimum number of sequences according to the table.

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EMI4_345_sm_FigS1.eps3578KSupporting info item
EMI4_345_sm_FigS2.eps3218KSupporting info item
EMI4_345_sm_FigureS3.tiff304KSupporting info item
EMI4_345_sm_material.doc133KSupporting info item

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