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Fig. S1. Detailed phylogenetic tree of actR genes obtained from cultures, single-cell amplified genomes and Lake Stechlin clone library. Sequences obtained from this study are highlighted in bold. For each sequence GC content is shown in a colour code, which ranges from 40% to 52%. The scale bar corresponds to 10 base substitutions per 100 nucleotide positions and bootstrap values above 50% are given.

Fig. S2. RNA gel was obtained by an automated electrophoresis system (Experion, Bio-Rad). Extracted RNA samples loaded on three RNA chips (RNA Standard Sens) according to the manufacturer's recommendations. After electrophoresis all RNA gel chips were combined and standardized and normalized by the size ladder and an internal standard at 50 nt, which was carried out automatically by the supplied software. Lanes marked by an asterisk are RNA preparations of water samples taken by the IFFS. Visible bands represent ribosomal RNAs of various sizes and of different origins. A potential degradation of RNA would appear as cleavage products of ribosomal RNAs in several fragments of lower size other than small rRNAs (not visible). Ratios of 23S to 16S were obtained by integrating their peaks in the corresponding chromatograms.

Fig. S3. Light intensity values (circles) obtained during the sampling day were combined with the expression pattern (box plots) of IFFS. Light values were kindly provided by Umweltbundesamt (Federal Environment Agency), measured by its monitoring station at Lake Stechlin.

Fig. S4. Technical drafts of IFFS can be assessed here [IFFS.zip]. All measurements are in millimetre.

Appendix S1. Primers and qPCR conditions for environmental actR expression analysis.

FilenameFormatSizeDescription
emi4350_sm_Figs1-3AppS1.doc3511KSupporting info item
emi4350_sm_Figs4.zip609KSupporting info item

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