Equal contributions to this article.
The existence and diversity of myxobacteria in lake mud – a previously unexplored myxobacteria habitat
Version of Record online: 22 AUG 2012
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Environmental Microbiology Reports
Volume 4, Issue 6, pages 587–595, December 2012
How to Cite
Li, S.-g., Zhou, X.-w., Li, P.-f., Han, K., Li, W., Li, Z.-f., Wu, Z.-h. and Li, Y.-z. (2012), The existence and diversity of myxobacteria in lake mud – a previously unexplored myxobacteria habitat. Environmental Microbiology Reports, 4: 587–595. doi: 10.1111/j.1758-2229.2012.00373.x
- Issue online: 14 NOV 2012
- Version of Record online: 22 AUG 2012
- Accepted manuscript online: 30 JUL 2012 07:21AM EST
- Manuscript Accepted: 22 JUL 2012
- Manuscript Received: 22 MAY 2012
- National Natural Science Foundation of China (NSFC) for Distinguished Young Scholars. Grant Number: 30825001
- NSFC Key Program. Grant Number: 31130004
Table S1. The primers used for the construction of 16S rRNA gene libraries and 454 pyrosequencing.
Table S2. Detailed diversity parameters of the four libraries.
Table S3. The bacterial community composition of the lake mud at the levels of phylum and order, determined by the libraries of UV34 and UV678.
Table S4. The OTU numbers of bacteria in phylum and order level at 3% phylogenetic distance.
Table S5. The composition of myxobacteria, calculated by bacteria universal libraries UV34 and UV678.
Table S6. The composition of suborder Cystobacterineae, calculated by Cystobacterineae enriched libraries CV34.
Table S7. The composition of suborder Sorangineae, calculated by Sorangineae enriched libraries SV678.
Table S8. Surveys of the appearance of myxobacteria-related sequences in the pyrosequencing data of lake sediments (released by He et al., 2012; retrieved from GenBank).
Supplementary materials S1. Isolation of myxobacteria strains from mud samples of Chenghai Lake.
Fig. S1-1. Colony photographs of Myxococcus (A) and Sorangium (B) strains isolated from Chenghai Lake mud. Bar in (A) is equal to 1.0 mm for 0558-ZXW144 and 0.5 mm for others. Bar in (B) is equal to 1.0 cm for So0558-31 and 0.5 cm for others.
Fig. S1-2. Phylogenetic analysis of the isolated myxobacteria stains based on 16S rRNA gene sequences. The bar is equivalent to two nucleotides changes per 100 bp. The numbers on branch nodes indicate bootstrap support percentages based on 1000 replications. The sequences from Chenghai Lake sediment are underlined for easy tracking.
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.