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emi4395-sup-0001-fS1.doc5101K

Fig. S1. Growth differences of P. annua seeds as a result of AMB overproduction in P. fluorescens. Strains (Table S1) were grown for 7 days in PMS medium ± 1 mM IPTG at 30°C. Cell-free culture supernatants were diluted in the same medium to 0.3× concentration and tested for GAF activity as described by Banowetz and colleagues (2008). Three surface-sterilized annual bluegrass seeds and 200 μl of test solution were placed in each well. Three replicate wells were set up for each treatment.

emi4395-sup-0002-fS2.doc3088K

Fig. S2. TLC analysis of P. fluorescens culture filtrates. Samples were treated and analysed as described in detail by Halgren and colleagues (2011). Briefly, culture filtrates were dried under vacuum, extracted with 90% ethanol and applied to Avicel Microcrystalline Cellulose TLC plates (Analtech, Inc.). Plates were developed over a distance of 12 cm in a mixture of ethylacetate/isopropanol/water (7.5/15/10), air-dried, and sprayed with a ninhydrin solution to visualize AMB (indicated by an arrow).

emi4395-sup-0003-tS1.doc92K

Table S1. Bacterial strains, plasmids and oligonucleotides.

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