The Pseudomonas aeruginosa antimetabolite L-2-amino-4-methoxy-trans-3-butenoic acid inhibits growth of Erwinia amylovora and acts as a seed germination-arrest factor
Article first published online: 8 OCT 2012
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Environmental Microbiology Reports
Thematic Issue on Environmental Ecology of Pathogens and Resistances
Volume 5, Issue 1, pages 83–89, February 2013
How to Cite
Lee, X., Azevedo, M. D., Armstrong, D. J., Banowetz, G. M. and Reimmann, C. (2013), The Pseudomonas aeruginosa antimetabolite L-2-amino-4-methoxy-trans-3-butenoic acid inhibits growth of Erwinia amylovora and acts as a seed germination-arrest factor. Environmental Microbiology Reports, 5: 83–89. doi: 10.1111/j.1758-2229.2012.00395.x
- Issue published online: 3 FEB 2013
- Article first published online: 8 OCT 2012
- Accepted manuscript online: 11 SEP 2012 04:52AM EST
- Manuscript Accepted: 30 AUG 2012
- Manuscript Revised: 22 AUG 2012
- Manuscript Received: 15 MAY 2012
- USDA-CSREES Grass Seed Cropping Systems for Sustainable Agriculture Special Grant Program
- OSU Agricultural Research Foundation
Fig. S1. Growth differences of P. annua seeds as a result of AMB overproduction in P. fluorescens. Strains (Table S1) were grown for 7 days in PMS medium ± 1 mM IPTG at 30°C. Cell-free culture supernatants were diluted in the same medium to 0.3× concentration and tested for GAF activity as described by Banowetz and colleagues (2008). Three surface-sterilized annual bluegrass seeds and 200 μl of test solution were placed in each well. Three replicate wells were set up for each treatment.
Fig. S2. TLC analysis of P. fluorescens culture filtrates. Samples were treated and analysed as described in detail by Halgren and colleagues (2011). Briefly, culture filtrates were dried under vacuum, extracted with 90% ethanol and applied to Avicel Microcrystalline Cellulose TLC plates (Analtech, Inc.). Plates were developed over a distance of 12 cm in a mixture of ethylacetate/isopropanol/water (7.5/15/10), air-dried, and sprayed with a ninhydrin solution to visualize AMB (indicated by an arrow).
Table S1. Bacterial strains, plasmids and oligonucleotides.
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