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Keywords:

  • Erosion;
  • demineralization;
  • dentine;
  • metalloproteinase;
  • soft drink

Abstract

Background:  Matrix metalloproteinase (MMP) inhibitors reduce dentine erosion. This in vitro study evaluated the effect of the supplementation of soft drinks with green tea extract, a natural inhibitor of MMPs, on their erosive potential against dentine.

Methods:  For each drink tested (Coca-Cola™, Kuat™ guarana, Sprite™ and light Coca-Cola™), 40 dentine specimens were divided into two subgroups differing with respect to supplementation with green tea extract at 1.2% (OM24®, 100%Camellia sinensis leaf extract, containing 30 ± 3% of catechin; Omnimedica, Switzerland) or not (control). Specimens were subjected to four pH cycles, alternating de- and remineralization in one day. For each cycle, samples were immersed in pure or supplemented drink (10 minutes, 30 mL per block) and in artificial saliva (60 minutes, 30 mL per block) at 37 °C, under agitation. Dentine alterations were determined by profilometry (μm). Data were analysed by two-way ANOVA and Bonferroni’s test (p < 0.05).

Results:  A significant difference was observed among the drinks tested with Sprite™ leading to the highest surface loss and light Coca-Cola™ to the lowest. Supplementation with green tea extract reduced the surface loss by 15% to 40% but the difference was significant for Coca-Cola™ only.

Conclusions:  Supplementation of soft drinks with green tea extract might be a viable alternative to reduce their erosive potential against dentine.