Effects of platelet-rich fibrin on human periodontal ligament fibroblasts and application for periodontal infrabony defects
Article first published online: 13 OCT 2011
© 2011 Australian Dental Association
Australian Dental Journal
Volume 56, Issue 4, pages 365–371, December 2011
How to Cite
Chang, Y.-C. and Zhao, J.-H. (2011), Effects of platelet-rich fibrin on human periodontal ligament fibroblasts and application for periodontal infrabony defects. Australian Dental Journal, 56: 365–371. doi: 10.1111/j.1834-7819.2011.01362.x
- Issue published online: 30 NOV 2011
- Article first published online: 13 OCT 2011
- (Accepted for publication 16 March 2011.)
- Platelet-rich fibrin;
- periodontal ligament fibroblasts;
- alkaline phosphatase;
- periodontal infrabony defects
Background: Platelet-rich fibrin (PRF) by Choukroun’s technique is derived from an autogenous preparation of concentrated platelets. Little is known about the effects of PRF on periodontal ligament fibroblasts (PDLFs) and the application of PRF for periodontal regeneration.
Methods: PDLFs were derived from healthy individuals undergoing extraction for orthodontic reasons. Blood collection was carried out from healthy volunteers. PRF was obtained from a table centrifuge centrifuged at 3000 rpm for 12 minutes. The effects of PRF on PDLFs were determined by measuring the expression of phosphorylated extracellular signal-regulated protein kinase (p-ERK), osteoprotegerin (OPG) and alkaline phosphatase (ALP) activity. Moreover, we retrospectively examined the feasibility and safety of reconstructing the periodontal infrabony defects with PRF in six patients.
Results: PRF was found to increase ERK phosphorylation and OPG in PDLFs in a time-dependent manner (p < 0.05). ALP activity was also significantly upregulated by PRF (p < 0.05). Application of PRF in infrabony defects exhibited pocket reduction and clinical attachment gain after six months. Periapical radiography revealed radiographic defect filled in grafted teeth.
Conclusions: The enhancement of p-ERK, OPG and ALP expression by PRF may provide benefits for periodontal regeneration. Clinical and radiologic analysis showed that the use of PRF is an effective modality for periodontal infrabony defects.