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Validation and Standardization of Virus Neutralizing Test Using Indirect Immunoperoxidase Technique for the Quantification of Antibodies to Rabies Virus

Authors

  • T. Ogawa,

    1.  National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, Tokyo, Japan
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  • K. Gamoh,

    1.  National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, Tokyo, Japan
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  • H. Aoki,

    1.  National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, Tokyo, Japan
    2.  Department of Basic Science, School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Tokyo, Japan
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  • R. Kobayashi,

    1.  National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, Tokyo, Japan
    2.  Animal Quarantine Service, Ministry of Agriculture, Forestry and Fisheries, Kanagawa, Japan
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  • M. Etoh,

    1.  National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, Tokyo, Japan
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  • M. Senda,

    1.  National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, Tokyo, Japan
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  • N. Hirayama,

    1.  Research Institute for Animal Science in Biochemistry and Toxicology, Kanagawa, Japan
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  • M. Nishimura,

    1.  Research Institute for Animal Science in Biochemistry and Toxicology, Kanagawa, Japan
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  • R. Shiraishi,

    1.  Research Institute for Animal Science in Biochemistry and Toxicology, Kanagawa, Japan
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  • A. Servat,

    1.  WHO Collaborating Centre for Research and Management in Zoonoses Control, OIE Reference Laboratory for Rabies, Community Reference Institute for Rabies Serology, Afssa site de Nancy, LERRPAS, Technopôle Agricole et Vétérinaire, Malzéville Cedex, France
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  • F. Cliquet

    1.  WHO Collaborating Centre for Research and Management in Zoonoses Control, OIE Reference Laboratory for Rabies, Community Reference Institute for Rabies Serology, Afssa site de Nancy, LERRPAS, Technopôle Agricole et Vétérinaire, Malzéville Cedex, France
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T. Ogawa. National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, 1-15-1, Tokura, Kokubunji, Tokyo 185-8511, Japan. Tel.: +81 0 423211841; Fax: +81 0 423211769; E-mail: ogawa@nval.go.jp

Summary

A virus neutralizing test using an indirect immunoperoxidase technique (VNT-IIP) for rabies has been developed for the titration of dog and cat serum samples in Japan. The VNT-IIP has the advantage that results obtained can be viewed by the naked eye. The purpose of this study was to validate the VNT-IIP and compare it with one of the international standard methods, the fluorescent antibody virus neutralization test (FAVNT). The VNT-IIP showed satisfactory repeatability, high analytical specificity and good accuracy. Regarding the comparison between the VNT-IIP and the FAVNT, the VNT-IIP showed good agreement (91.9%), high sensitivity (92.8%) as well as specificity (87.0%) and good correlation (r = 0.92). As described above, the validation of the VNT-IIP was satisfactory and the performances of the test proved to be equivalent to those of an international standard method.

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