These authors contributed equally to the study.
Use of a Bacterial Antimicrobial Resistance Gene Microarray for the Identification of Resistant Staphylococcus aureus
Article first published online: 17 NOV 2010
© 2010 Blackwell Verlag GmbH
Zoonoses and Public Health
Special Issue: SafePork Guest Editors: Lis Alban, Wondwossen Gebreyes, Sylvain Quessy, Andrijana Rajic, Peter van Der Wolf
Volume 57, Issue Supplement s1, pages 94–99, November 2010
How to Cite
Garneau, P., Labrecque, O., Maynard, C., Messier, S., Masson, L., Archambault, M. and Harel, J. (2010), Use of a Bacterial Antimicrobial Resistance Gene Microarray for the Identification of Resistant Staphylococcus aureus. Zoonoses and Public Health, 57: 94–99. doi: 10.1111/j.1863-2378.2010.01358.x
- Issue published online: 17 NOV 2010
- Article first published online: 17 NOV 2010
- Received for publication November 11, 2009
- Staphylococcus aureus;
- antimicrobial resistance;
As diagnostic and surveillance activities are vital to determine measures needed to control antimicrobial resistance (AMR), new and rapid laboratory methods are necessary to facilitate this important effort. DNA microarray technology allows the detection of a large number of genes in a single reaction. This technology is simple, specific and high-throughput. We have developed a bacterial antimicrobial resistance gene DNA microarray that will allow rapid antimicrobial resistance gene screening for all Gram-positive and Gram-negative bacteria. A prototype microarray was designed using a 70-mer based oligonucleotide set targeting AMR genes of Gram-negative and Gram-positive bacteria. In the present version, the microarray consists of 182 oligonucleotides corresponding to 166 different acquired AMR gene targets, covering most of the resistance genes found in both Gram-negative and -positive bacteria. A test study was performed on a collection of Staphylococcus aureus isolates from milk samples from dairy farms in Québec, Canada. The reproducibility of the hybridizations was determined, and the microarray results were compared with those obtained by phenotypic resistance tests (either MIC or Kirby-Bauer). The microarray genotyping demonstrated a correlation between penicillin, tetracycline and erythromycin resistance phenotypes with the corresponding acquired resistance genes. The hybridizations showed that the 38 antimicrobial resistant S. aureus isolates possessed at least one AMR gene.